荧光定量聚合酶链反应检测乙型肝炎病毒DNA及其与血清标志物的关系探讨  

Compare the relationship between hepatitis B virus DNA detected by fluorescence quantitative PCR and hepatitis B virus markers

作　　者：陈建森[1] 郑华燊[1] 卓光生[1] 曾德成[1] 

机构地区：[1]福建医科大学附属协和医院,350001

出　　处：《福建医药杂志》2002年第4期96-98,共3页Fujian Medical Journal

摘　　要：目的　用荧光定量聚合酶链反应 (fluorescence quantitative polymerase chain reaction,FQ- PCR)方法定量检测血清中乙型肝炎病毒 (hepatitis B virus,HBV)的数量及探讨其与 HBV标志物 (HBV M)表现模式的关系 ,以指导临床。方法　共 2 4 4份临床血清标本 ,HBV DNA定量采用荧光定量 PCR分析系统 ,HBV M采用 EL ISA法。结果　经 FQ- PCR检测 ,99例 HBs Ag(+) / HBe Ag(+) / HBc Ab(+)的标本 ,其血清标本 HBV DNA亦全部阳性 ,平均 HBV DNA拷贝数为 1.82× 10 7copies/ ml;96例 HBs Ag (+) / HBe Ab (+) / HBc Ab (+)的标本 ,其 HBV DNA检出率为 6 6 .7% (6 4例 ) ,平均拷贝数为 1.82× 10 4 copies/ m l;11例 HBs Ag (+) / HBc Ab (+)的标本其 HBV DNA检出率为 6 3.6 % (7例 ) ,平均拷贝数为 7.94× 10 4 copies/ ml。结论　血清 HBV DNA水平与 HBV M表现模式有关 ,HBs Ag (+) / HBe Ag (+) / HBc Ab (+)的标本 HBV DHA值显著高于 HBs Ag (+) / HBe Ab (+) / HBc Ab (+)的标本和 HBs Ag (+) / HBc Ab (+)的标本 ,提示 HBs Ag与 HBe Ag的存在影响 HBV DNA水平。 FQ- PCR能实现准确定量 ,可以检测 HBV的真实感染和复制情况 ,对于乙型肝炎的临床诊断。Objective To explore the relationship between hepatitis B virus DNA and hepatitis B virus markers.Methods 244 clinical serum samples were collected.Hepatitis B virus DNA was detected by FQ PCR and hepatitis B virus markers were detected by ELISA.Results In 99 HBsAg(+)/HBeAg(+)/HBcAb(+)samples,FQ PCR results were positive,with 1 82×10 7 copies/ml of HBV DNA average.In 96 HBsAg(+)/HBeAb(+)/HBcAb(+)samples,the average was 1 82×10 4 copies/ml with a positive rate of 66 4%.In 11 HBsAg(+)/HBcAb(+)samples,the average was 7 94×10 4 copies/ml with a positive rate of 63 6%.Conclusions There is a significant correlation between HBV markers and HBV DNA.The HBsAg(+)/HBeAg(+)/HBcAb(+)samples were significantly higher than the HBsAg(+)/HBeAb(+)/HBcAb(+)samples and the HBsAg(+)/HBcAb(+)samples.FQ PCR can be used to monitor the true state of HBV infection and amplification.

关 键 词：乙型肝炎病毒 DNA 肝炎病毒 定量PCR 

分 类 号：R446.6[医药卫生—诊断学]