聚合酶链反应(PCR)检测花鲈鳗弧菌感染  

Detection of Vibrio anguillarum from Lateolabrax japonicus by Using Polymerase Chain Reaction (PCR)

作　　者：余俊红[1] 陈吉祥[1] 厉云[1] 苟万里[1] 纪伟尚[1] 徐怀恕[1] 

机构地区：[1]青岛海洋大学海洋生命学院,山东青岛266003

出　　处：《黄渤海海洋》2002年第2期60-64,共5页Journal of Oceanography of Huanghai & Bohai Seas

基　　金：国家自然科学基金资助项目 (3 9870 5 81 )

摘　　要：从海洋鱼类重要病原菌———鳗弧菌基因库中选择金属蛋白酶基因为目标检测基因 ,以此设计一对引物 ,建立聚合酶链反应 (PCR)检测鳗弧菌的方法 ,并对此方法的灵敏性和特异性进行了研究 ,结果表明该方法对鳗弧菌的检测快速、灵敏。对人工感染鳗弧菌的花鲈组织样品进行检测 ,该方法能识别组织样品中极微量的鳗弧菌。Metalloprotease gene in gene bank of Vibrio anguillarum was selected as the target detection gene, a pair of primers was designed for detecting Vibrio anguillarum by using polymerase chain reaction (PCR), and the sensitivity and specificity of PCR method were studied. It is shown from the study results that the PCR method is quick and sensitive for detecting Vibrio anguillarum , and can detect trace Vibrio anguillarum from the tissue samples of Lateolabrax japonicus artificially infected with viable Vibrio anguillarum.

关 键 词：聚合酶链反应 鳗弧菌 金属蛋白酶基因 花鲈 

分 类 号：S943.211[农业科学—水产养殖]