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作 者:刘海青[1] 林瑞超[2] 冯芳[3] 党合群[1]
机构地区:[1]青海省药品检验所,西宁810000 [2]中国药品生物制品检定所,北京100050 [3]中国药科大学,南京210009
出 处:《药物分析杂志》2002年第5期392-395,共4页Chinese Journal of Pharmaceutical Analysis
摘 要:目的:建立测定卷柏类药材中双黄酮成分的高效液相色谱方法。方法:色谱柱:Elite-ODS(5 μm,150 mm×4.6mm);流动相:甲醇-0.050 mol·L^(-1)磷酸二氢钠溶液系统,梯度洗脱,0→6 min,甲醇52.5%→70.0%;柱温:40℃;流速:1.5 mL·min^(-1),检测波长:330 nm。结果:穗花杉双黄酮、扁柏双黄酮、银杏双黄酮加样回收率分别为97.05%,98.45%,96 88%(RSD=2.1%,2.8%,2.3%,n=3)。卷柏类药材均含有穗花杉双黄酮,但含量差异较大;另2种双黄酮成分在卷柏类药材中的分布与含量同样存在差异。结论:本法快捷、灵敏、准确,可作为评价卷柏类药材的参考依据。bjective: To establish a HPLC method for the determination of biflavones from herbs of Selaginella Spring. Methods: The column was Elite - ODS (5μm, 150 mm ×4. 6 mm) ; the mobile phase consisted of methanol and phosphate buffer, the gradient condition was 0→6 min, methanol 47. 5%→70. 0% ; the column temperature was 40℃ ; the flow rate was 1. 5 mL · min-1 and the detection was done at 330 nm. Results: The average recoveries of amentoflavone, hinokiflavone and ginkgetin were 97. 05% , 98. 45% and 96. 88% ( RSD = 2. 1% , 2. 8% , 2. 3% , n =3) respectively. Amentoflavone was a common component and its contents from 12 species of Selaginella Spring were markedly different. It also showed from HPLC the difference in distributions and contents of hinokiflavone and ginkgetin from Selaginella Spring. Conclusion: This method was simple, sensitive and rapid and was very suitable for the determination of biflavones.
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