RBL-2H3细胞诊断过敏性休克的可行性初探  

Study of diagnose guinea pigs anaphylaxis shock with RBL-2H3 cells

作　　者：郭薇[1] 陈玉川[1] 刘水平[1] 孔宪涛[2] 

机构地区：[1]中山医科大学法医病理教研室,广州510080 [2]上海长征医院实验科,上海200003

出　　处：《中国免疫学杂志》2002年第9期611-613,共3页Chinese Journal of Immunology

摘　　要：目的 :探索诊断过敏性休克的体外检测方法。方法 :建立豚鼠过敏性休克模型 ,培养RBL 2H3细胞株 ,用致敏动物血清在体外诱导该细胞脱颗粒 ,观察其形态改变及测定组胺释放量与细胞表面AnnexinV标记阳性率。结果 :RBL 2H3细胞在致敏血清刺激下活化 ,在抗原再次攻击后可以脱颗粒 ,AnnexinV阳性率与组胺释放量呈正比 ,二者与致敏血清的滴度呈剂量依赖关系。结论 :RBL 2H3可望用于过敏性疾病诊断及变应原筛选 。Objective:In order to study of diagnose anaphylactic shock in forensic field,a new in vitro testing sysytem were established.Methods:The RBL 2H3 were cultured and sensitized,the release of histamine and stained for Annexin V were testing by fluorescent spectrometer and flow cytometry respectively.Results:The degree of RBL 2H3 degranulation after sensitived serum treated was higher than that after control serum treated,and the degranulation degree increased dose dependently.Conclusion:RBL 2H3 are helpful to diagnose anaphylactic shock,and the release of histamine and the positive staining for Annexin V may be the ideal indices.

关 键 词：脱颗粒 ANNEXINV 组胺 RBL-2H3细胞 诊断 过敏性休克 

分 类 号：R593.1[医药卫生—内科学]