狼疮方对狼疮样小鼠肾脏RANTES表达的影响  

Ameliorate chemokine RANTES expression by the recipe of LangChangFang in murine lupus nephritis model

作　　者：梁鸣[1] 李幼姬[1] 阳晓[1] 魏毅[2] 叶任高[1] 许韩师[1] 周建中[1] 

机构地区：[1]中山医科大学附属第一医院肾内科卫生部肾脏病临床研究重点实验室,广州510080 [2]第一军医大学中医系,广州510080

出　　处：《中国免疫学杂志》2002年第9期631-635,共5页Chinese Journal of Immunology

基　　金：国家自然科学基金资助项目 (3 9970 93 3 );中山医科大学"2 11工程"重点科研基金资助项目 (9815 0 );广东省自然科学基金资助项目(990 0 73 )

摘　　要：目的 :观察慢性移植物抗宿主病 (GAHD)狼疮样肾炎小鼠模型肾组织中趋化因子RANTES的表达并探讨中药狼疮方对其的影响。方法 :以强的松为阳性对照 ,观察狼疮方对模型小鼠肾脏RANTES表达的影响 ,并进一步分析其对肾脏病理和功能损害的影响。结果 :狼疮方具有与强的松相似的防止和减轻狼疮样肾炎小鼠肾功能恶化、肾组织病理改变的作用(P <0 0 0 1)。这作用与减轻狼疮小鼠肾组织中RANTES表达显著相关。结论 :狼疮方明显下调狼疮肾炎肾脏RANTES表达 。Objective:To investigate chemokine RANTES(Regulated upon Activation.Normal T cell Expressed and Secreted)expression in the chronic graft versus host disease(GVHD) murine lupus nephritis model and effect of the recipe of LangChuangFang on it.Methods:A microwave based immunohistochemistry(SP) and RT PCR were used to detect the expression of RANTES in the kidney of model,therapeutic group and normal control.At the same time,the correlation between expression of RANTES and renal injures in model was examined.Results:LangChuangFang protected renal function,which was coincided with amelioration of pathologic lesions especially tubulointersitial injury.This was associated with the amelioration of RANTES protein and mRNA expression in model.Conclusion:RANTES involved in the process of renal injury in vivo,LangChuangFang can treat lupus as immunosuppressive agent by decreasing RANTES production.

关 键 词：移植物抗宿主病 狼疮性肾炎 中医药疗法 RANTES 狼疮方 

分 类 号：R259.932.4[医药卫生—中西医结合] R285.5[医药卫生—中医内科学]