慢性乙型肝炎患者外周血树突细胞CD40、CD80表达和免疫活性的体外研究  

Study of CD40 and CD80 on peripheral blood dendritic cells in patients with chronic hepatitis B in vitro

作　　者：周雨迁[1] 詹俊[1] 邓庆丽[1] 智慧[2] 魏菁[1] 黄志明[1] 

机构地区：[1]广州中山医科大学孙逸仙纪念医院消化内科,广州510120 [2]中山医科大学孙逸仙纪念医院超声科

出　　处：《中国免疫学杂志》2002年第9期639-642,共4页Chinese Journal of Immunology

基　　金：97 3分课题资助项目 (G1990 5 410 5 )

摘　　要：目的 :探讨慢性乙型肝炎患者DC表面协同刺激分子CD4 0和CD80表达与其抗原呈递功能的关系 ,为进一步研究慢性乙型肝炎的发病机制及治疗提供新的思路。方法 :分离培养慢性乙型肝炎患者和健康人外周血DC ,流式细胞仪测定DC表面CD4 0和CD80的表达 ,混合淋巴细胞反应测定两组DC的功能。结果 :慢性乙型肝炎患者DC表面CD4 0和CD80的表达较健康人低 ,刺激同种异种T细胞增殖反应也较健康人低。结论 :乙肝病毒感染可通过抑制患者DC表面CD4 0、CD80等协同刺激分子的表达 。Objective:To study the relation between the costimulating molecules CD40,CD80 on DC and their antigen presentation function in patients with chrnic HBV infection .Methods:The DC from healthy donors and patients were stained with anti human specific mouse monoclonal antibodies conjugated with fluorescein isothiocyanate(FITC) respectively.Mouse McAb used were anti HLA ABC,HLA DR,CD1a,CD14,CD40,CD80.Results:There are no remarkable difference in MHC I?MHC II between two groups;remarkable difference was showed in CD40(P<0.01) and CD80 expression(P<0.05),both are lower in patients than in the health's.Both of two groups can stimulate vigoroous proliferation of allogenic lymphocytes,and the reaction is stronger with the DC number increasing,When DC numbers are the same,proliferation of lymphocytes from healthy donors is obviously stronger than that of the patients.Conclusion:The costimulating molecules CD40 and CD80 are lower in patients' DC than those in the healthy,which may lead to dysfuction of DC in these patients.

关 键 词：慢性乙型肝炎 外周血 树突细胞 CD40 CD80 免疫活性 体外研究 

分 类 号：R512.62[医药卫生—内科学]