牛病毒性腹泻病毒侵染宿主细胞的电镜观察  

Electron Microscopic Studies of Bovine Viral Diarrhea Virus in Cell Cultures

作　　者：王新华[1] 刘守仁[1] 张荣兴[2] 葛锡锐[2] 

机构地区：[1]新疆农垦科学院,石河子832000 [2]中国科学院上海细胞生物研究所,上海200031

出　　处：《畜牧兽医学报》2002年第5期501-503,共3页ACTA VETERINARIA ET ZOOTECHNICA SINICA

摘　　要：用电镜观察了牛病毒性腹泻病毒OregonC2 4 株在侵染新生犊牛睾丸细胞中的形态发生。成熟的病毒颗粒呈直径约为 5 0nm的球形颗粒 ,内含直径约为 30nm的核芯。病毒侵染宿主细胞后 ,在胞质内复制 ,通过糙面内质网膜出芽成熟。病毒可通过侵染细胞外排或在细胞死亡后含有病毒颗粒的空泡破溃而释放到胞外。The morphogenesis of bovine viral diarrhea virus (BVDV) strain Oregon C24 in bovine testis cell culture was examined by electron microscopy.Virions were roughly round and approximately 50 nm in diameter with a 30 nm core.Virus replication took place within cytoplasm of host cells.Budding of BVDV through membrane of modified rough endoplasmic reticulum was observed.The virus might be released by exocytosis or by disintegration of the vacuoles containing virus after cell death.

关 键 词：牛 病毒性腹泻病毒 宿主细胞 电镜观察 出芽过程 

分 类 号：S852.659.6[农业科学—基础兽医学]