鸡新城疫疫苗点眼免疫后哈氏腺中IgA、IgM、IgG型浆细胞的数量变化及分布特点  

Changes and Distribution of Plasma Cells After Eye-drop Vaccination Against Chicken Newcastle Disease

作　　者：王彩虹[1] 高齐瑜[1] 孙斌[2] 

机构地区：[1]中国农业大学动物医学院,北京100094 [2]黑龙江八一农垦大学动物科技学院,密山158308

出　　处：《畜牧兽医学报》2002年第5期504-507,共4页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金资助项目 ( 39370 5 33)

摘　　要：用Lasota系ND疫苗分别于 10、2 0日龄进行点眼免疫后采用SLAB免疫组织化学方法对鸡哈氏腺在鸡新城疫 (NewcastleDisease ,ND)疫苗点眼免疫后IgA、IgM、IgG型浆细胞的数量变化及分布特征进行了研究。研究发现 ,Lasota系ND疫苗点眼免疫后鸡哈氏腺中 (HarderianGland ,HG)中IgA、IgM、IgG型浆细胞的出现较对照组早 ,并于 48日龄达到峰值 ,一免及二免后三种类型的浆细胞数量均极显著高于对照组 (P <0 0 1) ,其中IgA型浆细胞的数量最多 ,IgM型浆细胞次之 ,IgG型浆细胞的数量最少。IgA、IgM、IgG型三种浆细胞均散在分布于哈氏腺的间质中 ,且主要围绕各级管腔上皮及腺上皮分布 ,其中成熟的浆细胞紧贴腺上皮及管腔上皮分布。表明Lasota系ND疫苗点眼免疫后可促进哈氏腺中的IgA、IgM、IgG型浆细胞的分化、成熟 ,这些浆细胞分泌的抗体成分可通过上皮而被分泌到管腔中去 ,最后到达泪液中 ,在眼区及上呼吸道起到保护作用。Changes and distribution of plasma cells after eye-drop vaccination against chicken Newcastle Disease were investigated.Results showed that IgA?IgM?IgG-secreting cells appeared earlier than those of the untreated group,and the amount of the three type cells were significantly higher than that of the control group(P<0.01).Among the three type cells,IgA-secreting cells were the most,IgG-secreting cells were the least.All of the three type cells were distributed around the epithelium of the duct and acini.It was suggested that the differentiation of the IgA?IgM?IgG-secreting cells in the Harderian Gland could be improved by the eye-drop vaccination with ND vaccine and the Harderian Gland played an important role during the eye-drop immunization.

关 键 词：鸡新 城疫疫苗 点眼免疫 哈氏腺 IgA型浆细胞 IgM型浆细胞 IgG型浆细胞 数量变化 分布特点 

分 类 号：S858.315.3[农业科学—临床兽医学] S858.312.5[农业科学—兽医学]