肝硬化大鼠部分肝切除后TGF-α和C-met蛋白在肝脏中的表达及其意义  

Expression and significance of TGF-α and C-met protein in the liver after partial hepatectomy in cirrhotic rats

作　　者：赵峰[1] 徐文[1] 陈栋[2] 吴立群 杨金镛[3] 

机构地区：[1]山东省临沂市人民医院普外科,山东临沂276003 [2]华中科技大学同济医学院器官移植研究所,湖北武汉430030 [3]青岛大学医学院附属医院肝胆外科,山东青岛266001

出　　处：《中国现代普通外科进展》2002年第3期165-167,共3页Chinese Journal of Current Advances in General Surgery

摘　　要：目的 :研究转化生长因子 α(TGF α)、C met蛋白在肝硬化大鼠肝脏中的表达及意义。方法 :将成年雄性Wistar大鼠制作为肝硬化大鼠 ,然后将肝脏部分切除制作肝再生模型。随机分为 7组 ,一组立即处死 ,计算肝切除率 ;其他组分别于术后 12h、1d、3d、5d、7d、14d处死。用免疫组织化学方法检测TGF α和C met蛋白在肝细胞中的表达 ,以正常大鼠肝再生模型为对照。结果 :肝硬化大鼠部分肝脏切除后不同时间肝中TGF α、C met蛋白表达的变化均较正常大鼠延迟。结论 :TGF α和C met在肝脏中的表达显示 ,硬化肝脏具有再生能力 ,但较正常肝脏弱。Objective:To investigate the expression of Transforming Growth Factor alpha(TGF α)and C met protein in regenerated liver in cirrhotic rats.Methods:We made adult male Wistar rats into cirrhotic rats,and dissected the left and middle lobes of the liver.Randomized these rats into seven groups.One group was sacrificed immediately after hepatectomy,calculated the rate of dissection.The other groups were sacrificed at 12 th hour,1 st day,3 rd day,5 th day,7 th day,and 14 th day after hepatectomy.We examined the expressions of TGF α and C met protein with immunohistochemical method(SABC).We used the model of liver regenertion in normal rats as the control groups.Results:The expressions of TGF α and C met were both change slower than the normal rats.Conclusion:The liver in cirrhotic rat has the capacity of regeneration.But the capacity of liver regeneration in cirrhotic rat is weaker than the normal rat.

关 键 词：肝硬化 大鼠 肝切除 转化生长因子Α 基因产物 C-met 基因表达 

分 类 号：R657.31[医药卫生—外科学]