组织工程化人工神经修复周围神经缺损的实验研究  

An experimental study on repair of peripneyal nerve defects with tissue engineering artificial nerve

作　　者：王光林[1] 杨志明[1] 林卫[2] 毕建红[3] 李胜富[4] 张杰[5] 

机构地区：[1]四川大学华西医院骨科,成都610041 [2]华西二医院妇产科 [3]四川大学基础医学院免疫教研室 [4]四川大学华西医院移植免疫室 [5]四川大学华西医院电镜室

出　　处：《中华手外科杂志》2002年第3期134-137,共4页Chinese Journal of Hand Surgery

基　　金：国家自然科学基金资助项目 ( 30 10 0 190 );四川省卫生厅基金资助项目 ( 0 0 0 0 2 8)

摘　　要：目的　研究雪旺细胞和生物降解支架材料复合后构建成的组织工程化人工神经修复神经缺损的效果。方法　将雪旺细胞制成 1× 1 0 8/ml的ECM (extracellularmatrix,ECM)凝胶 ,与PLA无纺纤维布复合培养 7d ,置入PLA中空纤维管中 ,构建成组织工程化人工神经。建立大鼠坐骨神经缺损 1 0mm的动物模型 ,A组为人工神经组 ;B组为雪旺细胞复合ECM凝胶组 ;C组为单纯ECM凝胶组 ;D组为自体神经移植组。术后 8周、1 2周 ,行神经电生理和组织学检测评价疗效。结果　术后 1 2周A组的再生神经纤维已越过远端缝合口 ,有髓神经纤维数和神经纤维密度稍差于D组 ,但再生神经组织的面积显著高于后者 ;A、D组的髓鞘厚度和dLAT、NCV、AMP、AREA均无显著差别 ,但明显高于B、C组。结论　雪旺细胞、ECM凝胶和PLA多孔材料与PLA中空纤维管组合后构建成的组织工程化人工神经 。Objective To study the outcome of repair of nerve defects with tissue engineering artificial nerve, which was composed of compound of Schwann cells and biodegradable materials.Methods Extracelluar matrix(ECM) gel (1×10 8/ml ) was fabricated from Schwann cells, which were co cultured with PLA steroframes for 7 days, and implanted into PLA conduits to construct tissue engineering bioartificial nerve . Model of sciatic nerve defects 10mm in length was set up. The animals were divided into 4 groups; artificial nerve group (A),compound of Schawann cells and ECM gel(B), pure ECM gel group(C),autogenous nerve transfer group(D).Evaluation of electrophysiological and histological results was carried out 8 and 12 weeks after operation.Results The regenerating nerve fibers in group A were grown into the distal nerve anastomoses at postoperative 12 weeks. The total myelinated fibers and density of the myelinated fibers in group A were slightly inferior to that of group D .But the regenerated nerve area in group A was larger than that in group D. There was no significant difference in the myelin thickness of sheath and dLAT ?NCV?AMP?AREA between group A and D , but those in group A and D were significantly higher than those in group B and C. Conclusions The tissue engineering bioartificial nerve with compound of Schwann cells,ECM gel,PLA poly hole material and PLA hollow fiber conduit had treatment outcome approximating to that of the nerve autograft for repair of periphral nerve defects.

关 键 词：组织工程化人工神经 修复 周围神经缺损 实验研究 

分 类 号：R651.3[医药卫生—外科学]