He-Ne激光加速兔骨折愈合的生物学效应及机理探讨  

BIOEFFECT AND DISCUSSION ABOUT MECHANISM OF HE-NE LASER IRRADIATION STIMULATING FRACTURE HEALING

作　　者：陈建平[1] 谭润初[1] 李济时[1] 

机构地区：[1]中山医科大学物理教研室

出　　处：《中山医科大学学报》1991年第4期265-271,327,共7页Academic Journal of Sun Yat-sen University of Medical Sciences

摘　　要：将39只健康雄性新西兰白兔左桡骨人工骨折,利用两种不同剂量(6.24J/cm^2和31.2J/cm^2)的He-Ne激光直接照射骨折区,摄X线平片获取骨愈合信息,平行测定血红细胞浓度、血红蛋白含量、血清碱性磷酸酶活性、血清钙浓度和血清羟脯氨酸浓度5项血液生化指标,并在骨愈合后测定其生物力学性能参数,结果表明:①激光照射使血清钙和血清羟脯氨酸浓度升高,血红细胞浓度降低,②6.24J/cm^2激光照射对血清碱性磷酸酶活性没有影响,而31.2J/cm^2激光照射却抑制了血清碱性磷酸酶活性,③6.24J/cm^2激光照射降低了血红蛋白含量,而31.2J/cm^2激光照射却对血红蛋白含量。没有影响;④生物力学测试表明,愈合骨拉伸性能没有改善,而弯曲性能则有所改善。两种剂量的照射均能促进骨折的愈合,但6.24J/cm^2照射的刺激效应似乎更为明显。The artificial fractures were on the left radius of 30 healthy male New Zealand white rabbits. Two levels of He-Ne laser energy density (6.24J/cm2 and 31.2J/cm2) were used for irradiating the area of fracture. Five biochemical indexs in the blood of the rabbits were determind during the experiment, they are the concentration of red blood cell and hemoglobin, the activity of serum alkaline phosphatase and the concentration of serum calcium and serum hydroxyproline. Fracture healing was evaluated with X-ray. Some biomechanical parameters of the bone were alse tested after the clinical union of the bones. The experimental results showed that the laser irradiation would induce an increase in the concentration of serum calcium and hydroxyproline, and a decrease in the concentration of red blood cell. The activity of serum alkaline phosphatase was inhibited by the irradiation of 3l.2J/cm2 but not affected by the irradiation of 6.24J/cm2. While the concentration of hemoglobin was decreased by the irradiation of lower level but not affected by the higher level one. In the biomechamical testing, it was found that the flexibility of the clinically union bone was improved while the tensility was not. Generally, both levels of the laser irradiation could stimulate the fracture healing, but the effect of the lower level one seems stronger.

关 键 词：骨折 激光 生物效应 红细胞 血清钙 

分 类 号：R68[医药卫生—骨科学]