不同海拔地区Wistar大鼠红细胞2,3-二磷酸甘油酸含量的比较  

THE CHANGES OF RBC 2,3-DIPHOSPHOGLYCENIC ACID LEVELS IN WISTAR RATS AT DIFFERENT ALTITUDE

作　　者：佘海茹 格日力 陈秋红 阮宗海 

机构地区：[1]青海省心血管病医院,810012

出　　处：《高原医学杂志》2000年第1期33-35,共3页Journal of High Altitude Medicine

摘　　要：探讨在缺氧环境下 ,移居高原的平原动物Wistar大鼠红细胞 2 ,3-二磷酸甘油酸 2 ,3-DPG浓度的变化 ;方法 :将Wistar大鼠在海拔 2 2 6 0m和 42 80m地区分别饲养 40天后 ,采用西德Bochniger-Mannheim公司生产的试剂盒 ,利用紫外分光光度法 ,对大鼠红细胞 2 ,3-DPG含量进行了测定 ;结果 :在 42 80m地区大鼠红细胞 2 ,3-DPG浓度为 (5 .83± 1 .33)nmol/L ,2 2 6 0m地区为 (4 .85± 0 .74)nmol/L ,经统计学处理P <0 .0 1。同时还对玛多返回西宁 40天后Wistar大鼠红细胞 2 ,3-DPG含量做了动态观察 ,发现随着海拔高度的下降 ,2 ,3-DPG的浓度也相应地下降 ,(4 .86± 0 .84)nmol/L ;结论 :随着海拔高度的增加 ,大鼠红细胞 2 ,3-DPG浓度的适当增加 ,是机体对缺氧的一种代偿性适应性改变。To study changes of RBC 2,3-diphosphoglycenic acid (2,3 DPG)in rats exposed to high altiude from sea level.Methods:The 35 Wistar rats were selected in the study.After they were fed seperataly for 40 days at 2 260m and 4 280m of altitude,level of RBC 2,3-DPG was measured using a kit, made in Bochniger-Mannheim co. German,by ultroviolet sectrophotomery.Results:The level of 2,3-DPG in rats at 4 280m altitude (5.83nmol/L±1.33 nmol/L) was higher (P<0.01) than that at 2 260m (4.85nmol/L±0.74nmol/L).And the level of 2,3-DPG in 9 of 22 rats reurned to 2 260m from 4 280m altitude was found to decrease after 40 days back come.Conclusions:It is an adaptive tress response to high altitude hypoxia in animals that the level of 2,3-DPG increases with elevating altitude.

关 键 词：红细胞 2 3-二磷酸甘油酸 高原地区 紫外分光光度法 缺氧环境 急进高原适应 

分 类 号：R188[医药卫生—流行病学]