中药材鹿鞭的分子鉴定研究  

Research on the Identification of Penis et Testis Cervi with Molecular Taxonomy

作　　者：唐双焱[1] 傅文[1] 陈永久[2] 王建云[1] 蒋序[1] 张亚平[2] 

机构地区：[1]云南省药品检验所,云南昆明650011 [2]中国科学院昆明动物研究所,云南昆明650223

出　　处：《中国中药杂志》2002年第8期573-575,共3页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金资助 (3 9760 0 81) ;云南省应用基础研究基金 (97C0 71M )

摘　　要：目的 :建立中药材鹿鞭的分子分类学鉴定试剂盒。方法 :根据对不同产地梅花鹿、马鹿、白唇鹿、水鹿线粒体DNA进行PCR扩增和序列测定 ,并与常见伪充药材来源动物线粒体DNA同位置序列比较 ,找到该 4个鹿种的特征片段 ,经过实际检验 ,筛选出合适片段作为鹿的种属特异性PCR引物。结果 :该引物与相关试剂组成试剂盒后 ,可用于中药材鹿鞭与常见伪充药材牛鞭、驴鞭等的鉴别。结论 :用分子分类学方法鉴别中药材鹿鞭 ,具有科学、稳定、准确、简便等特点 ,值得推广。Objective: To make the kit with witch to identify Penis et Testis Cervi with molecular taxonomy. Method: The mtDNA of sika and red deer from different areas was amplified by PCR and sequenced. Compared with the mtDNA of bovine and horse from witch the false medicines were made, characteristic segments of deer were found. We selected one as the species distinctive PCR primer of deer. Result: The kit made up with this primer and related reagents could be used to discern Penis et Testis Cervi from the false medicine. Conclusion: It is a scientific, steady, accurate and convenient way to identify Penis et Testis Cervi with molecular taxonomy.

关 键 词：鹿鞭 分子分类 线粒体DNA 种属特异性引物 PCR扩增 中药鉴定 

分 类 号：R282.740.3[医药卫生—中药学]