纤维蛋白原基因多态性与缺血性心脑血管病的关系  

The relationship of beta-fibrinogen gene polymorphisms and ischaemic cardiocerebral vascular disease

作　　者：刘蓉[1] 李家增[1] 穆红[1] 江雁[1] 王玉亮[1] 党群[1] 崔娴维 汲淼 黄繁嫱[1] 

机构地区：[1]天津市第一中心医院,天津市血栓与止血研究所300192 [2]解放军第二○二医院检验科

出　　处：《中华血液学杂志》2002年第9期453-456,共4页Chinese Journal of Hematology

基　　金：国家自然科学基金资助项目 ( 39830 180 )

摘　　要：目的　调查健康人、心肌梗死患者及脑梗死患者的纤维蛋白原 (Fg) β - 45 5G/A、- 148C/T、44 8G/A基因多态性频率分布、Fg分子反应性及与血浆Fg水平的关系。方法　用限制性片段长度多态性分析基因频率分布 ,用计算机辅助的纤维蛋白单体聚合反应分析方法和Clauss法分析血浆Fg水平。结果　等位基因 - 45 5A、- 148T和 44 8A在正常人中的频率分别是 0 .185 ,0 .194及 0 .192 ;在心肌梗死患者中的频率分别是 0 .2 95 ,0 .318及 0 .30 7;在脑梗死患者中的频率分别是 0 177,0 .193及 0 .182。心肌梗死患者中 - 45 5A、- 148T和 44 8A的频率比健康人明显升高。 3个多态性位点 - 45 5G、- 148C和44 8G或 - 45 5A、- 148T和 44 8A分别紧密连锁 ,符合率超过 98%。心脑血管病患者的Fg功能明显增高且与Fg水平相关。 3个多态性位点不同基因型组血浆Fg水平差异无显著性。结论　 3对等位基因紧密连锁不平衡 ,不同基因型组血浆Fg水平差异无显著性 ,心肌梗死患者中 - 45 5A、- 148T和 44 8A的频率比健康人明显升高。提示Fgβ - 45 5G/A、- 148C/T和 44 8G/A三种基因多态性与血浆Fg水平无关 ,而与心肌梗死的发病相关。心脑血管病患者不仅Fg功能明显增高 。Objective To analyze the frequency of beta-fibrinogen (β-Fg) gene -455G/A,-148C/T and 448G/A polymorphism, fibrinogen molecular reactivity and their association with plasma fibrinogen levels in health adults, myocardial infarction and cerebral infarction disease. Methods The β-Fg gene -455G/A,-148C/T and 448G/A polymorphisms were analyzed by restriction fragment length polymorphism (RFLP). Fibrinogen molecular reactivity was analyzed for the conversion kinetics of fibrinogen into fibrin by a computer assistant procedure. Plasma fibrinogen levels were determined by Clauss method. Results The frequencies of -455A, -148T, 448A allele in health adults were 0.185, 0.194 and 0.192, in myocardial infarction disease 0.295, 0.318 and 0.307, in cerebral infarction disease 0.177, 0.193 and 0.182, respectively. The frequencies of -455A, -148T, 448A alleles in myocardial infarction disease were apparently higher than that of health adults. There were close linkage between -455G, -148C and 448G or -455A, -148T and 448A, the correspondence was over 98%. There are no differences in the plasma fibrinogen levels of the three polymorphisms in two genotype groups. The fibrinogen molecular reactivity was significantly increased in cardiocerebral vascular disease and related with plasma fibrinogen level. Conclusion The three polymorphisms loci are strong linkage disequilibrium. There are no significant differences in the plasma fibrinogen levels of the three polymorphisms in two genotype groups. The frequencies of -455A, -148T, 448A alleles in myocardial infarction disease were apparently higher than that of health adults. It suggest that there was no association between β-Fg gene -455G/A,-148C/T and 448G/A polymorphisms and plasma fibrinogen levels, but did in myocardial infarction disease. The fibrinogen molecular reactivity was significantly increased in cardiocerebral vascular disease and related with plasma fibrinogen level.

关 键 词：病理 纤维蛋白原基因多态性 缺血性脑血管病 心肌梗死 脑梗死 

分 类 号：R542.22[医药卫生—心血管疾病] R743[医药卫生—内科学]