荧光定量PCR检测不明原因流产组织及血清中巨细胞病毒和弓形体感染  

Fluorescent quantitative PCR(FQ-PCR) detect the infection of CMV and TOX in decidua ,villus and serum in women suffered unexplained abortion.

作　　者：张宁[1] 鲁海鸥[1] 闫素文[1] 赵欣[1] 王梦醒[1] 

机构地区：[1]解放军第202医院全军优生优育研究中心,110003

出　　处：《中国优生与遗传杂志》2002年第4期34-35,共2页Chinese Journal of Birth Health & Heredity

摘　　要：目的　探讨用荧光定量PCR检测不明原因流产组织及血清中巨细胞病毒 (CMV)和弓形体 (TOX)感染。方法　检测了 6 6名妊娠 70天内流产患者的绒毛和蜕膜组织中CMV、TOX ,探讨了与血清中IgM抗体和DNA拷贝数的关系。结果　不明原因流产者查出血清CMV感染者 6例 (9 1% ) ,其中血清、蜕膜和绒毛均感染者有 3例占 (4 5 % ) ;1例血清、蜕膜感染但未采到绒毛标本 ;2例 (3 0 % )母血清中查到CMV ,流产组织中未查到。查出血清、蜕膜和绒毛TOX均感染者 1例。FQ -PCR检测出CMV阳性者 5例 ,TOX 1例 ,而IgM无 1例阳性。当流产妇女外周血CMV -DAN≥ 10 3 时 ,有 6 7%在流产组织中查到CMV -DAN。结论　荧光定量PCR在血清、脱膜和绒毛组织检测中显现的高敏感和高特异性 ,应推荐作为妊娠初期筛查的首选方法。Objective: Detect the infection of CMV and TOX in tissue ,serum of unexplained abortion by FQ-PCR. Methods: We evaluated the DNA copy of CMV and TOX in decidua and villus from 66 women suffered abortion within 2 weeks.We also explored the relationship between IgM antibody in serum and DNA copy. Results: We found 6 women showed CMV infection(9.1%), among which there were 3 women showed same infection in serum, decidua and villus(4.5%), 1 women showed serum and decidua infection but no villus sample, 2 women (3.0%) showed infection in serum but not in abortion tissue. One TOX infection was found in serum ,dedidua and villus.5 women showed CMV positive and 1 women showed TOX positive by FQ-PCR but no positive result of IgM antibody. Nearly 67% abortion women can be found CMV infection in abortion tissue when CMV-DNA ≥10 3 in serum. Conclusion: FQ-PCR can be recommended as first-choice method screening infection during first trimester pregnancy because of its sensitivity and speciality in evalution of serum,dedidua and villus infection.

关 键 词：荧光定量PCR 蜕膜 绒毛 巨细胞病毒 弓形体 流产 

分 类 号：R714.21[医药卫生—妇产科学]