鲁梅克斯K-1杂交酸模愈伤组织诱导及细胞悬浮系的建立  

Study on Callus Induction and Cell Suspension Line of Hybrid Sour Dock cv.Rumex K-1

作　　者：杨起简[1] 周禾[2] 于同泉[1] 张蕾[1] 

机构地区：[1]北京农学院农学系,北京102206 [2]中国农业大学草地所,北京100094

出　　处：《华北农学报》2002年第3期75-79,共5页Acta Agriculturae Boreali-Sinica

基　　金：北京市自然科学基金资助项目 (6 992 0 7)

摘　　要：研究了不同激素组合处理对鲁梅克斯K 1杂交酸模子叶愈伤组织的诱导 ,试验结果表明 ,2 ,4 D配合使用 6 BAP时愈伤组织诱导率最高。在附加 1 0mg/L 2 ,4 D的液体MS培养基中通过悬浮培养分离出的单细胞 。This paper studied the effects of different hormone combinations and concentrations on callus induction hybrid sour dock cv.Rumex K 1.The experimental results showed that the induced rate of callus was the best by MS medium supplemented with 2,4 D and 6 BAP.The monocells were cultured in MS liquid medium supplemented with 1 0 mg/L 2,4 D was appropriate.After some times of culture,the cells suspension culture was set up.

关 键 词：鲁梅克斯K-1杂交酸模 愈伤组织 诱导 细胞悬浮系 

分 类 号：S54[农业科学—作物学]