C肽对12周STZ大鼠糖尿病肾病的干预作用  

Prevention of Diabetic Nephropathy in 12 Week STZ Induced Diabetic Rats Treated by C-peptide

作　　者：孙蔚[1] 高鑫[1] 崔大敷 夏其昌[2] 

机构地区：[1]复旦大学附属中山医院内分泌科,上海200032 [2]中国科学院上海生物化学研究所,上海200031

出　　处：《复旦学报（医学版）》2002年第5期338-342,共5页Fudan University Journal of Medical Sciences

基　　金：上海市现代生物与新药产业发展基金资助 (9843 1912 0 )

摘　　要：目的　应用STZ大鼠模型探讨单独应用C肽对糖尿病肾病的干预作用。方法　大鼠分 4组 :(1)血糖控制接近正常的糖尿病胰岛素强化组 (DM I组 ) ;(2 )维持高血糖状态的糖尿病C肽 (130nmol/kg每天两次皮下注射 )组 (DM C组 ) ;(3)血糖水平与DM C组一致的糖尿病组 (DM组 ) ;(4)同龄正常大鼠为对照组 (N组 )。12周后测定肾重 /体重比值、肾小球体积、细胞外基质 /肾小球截面积比值、肾小球基底膜厚度和尿白蛋白排泄率。结果　DM C组的肾小球体积与细胞外基质 /肾小球截面积比值达到DM I组和N组水平 ,约为DM组相应参数的 5 1%与 74 % (P <0 .0 0 1)。DM C组的肾重 /体重比值与尿白蛋白排泄率接近DM组 (P >0 .0 5 ) ,高于DM I组 (分别为P =0 .0 3与P =0 .0 5 )。各组肾小球基底膜厚度无统计学差异。结论　单独应用C肽 12周可以预防STZ大鼠肾小球细胞外基质积聚导致的系膜扩张和肾小球肥大 ,且不依赖糖代谢紊乱的纠正 ,但对整个肾脏肥大和尿白蛋白漏出没有预防作用。Purpose. To investigate the prevention effect of C-peptide on diabetic nephropathy in STZ rats. Methods. The study included 4 groups of rats: (1) intensive treated diabetic group (DM-I group) with nearly normal blood glucose level; (2) C-peptide (130 nmol/kg, sc, twice daily) diabetic group (DM-C group) with constant high blood glucose level; (3) non-treated diabetic group (DM group) with the same blood glucose level as DM-C group; (4) age matched non-diabetic control rats (N group). After 12 weeks of diabetes, ratio of kidney weight/body weight(KW/BW), glomerular volume [V(glom)], extracellular matrix area fraction of glomerular cross-section [S(ECM)/S(glom)], glomerular basement membrane thickness (GBMT) and urinary albumin excretion rate (UAER) were measured. Results. DM-C group had V (glom) and S(ECM)/S(glom) similar to those of DM-I group and N group, which were approximately 51% and 74% respectively of those in DM group (P0.05) while increased compared to DM-I group (P = 0.03 and 0.05 respectively). GBMT of all groups had no statistical difference. Conclusions. The accumulation of glomerular extracellular matrix which leads to mesangial expansion and glomerular hypertrophy can be completely prevented by 12 week C-peptide treatment independent of metabolic control in STZ rats. But there is no prevention effect on whole kidney hypertrophy and leakage of urinary albumin.

关 键 词：糖尿病肾病 C肽 胰岛素 肾脏形态学 尿白蛋白排泄率 

分 类 号：R587.2[医药卫生—内分泌] R692[医药卫生—内科学]