新型铕络合物用于HBsAg的时间分辨荧光免疫检测  

Time-resolved Fluoroimmunoassay for Hepatitis B Surface Antigen Using a New Europium Fluorescent Chelate

作　　者：朱艳冰[1] 李庆阁[1] 王桂兰[2] 袁景利[2] 

机构地区：[1]厦门大学生命科学学院细胞生物学与肿瘤细胞工程教育部重点实验室,厦门361005 [2]中国科学院大连化学物理研究所,大连116012

出　　处：《标记免疫分析与临床》2002年第3期157-160,共4页Labeled Immunoassays and Clinical Medicine

摘　　要：利用稳定的新型铕荧光络合物BHHCT与Eu3 + 标记羊抗人HBsAb和Eu3 + 标记BSA -SA ,建立定量测定血清HBsAb的Eu3 + -BHHCT -HBsAb -TRFIA法和Eu3 + -BHHCT -BSA -SA -TRFIA法。结果表明 ,这两种方法最低检出值分别为 0 .2ng/mL和 0 .0 5ng/mL ,标准曲线范围均为 0 - 10 0ng/mL ,批内变异系数CV均小于10 % ,后者回收率为 85 % - 115 %。用Eu3 + -BHHCT -BSA -SA -TRFIA法与ELISA法同时检测 118份血清样品 。A new and stable fluorescent europium chelate Eu 3+ -BHHCT was used as a label to develop two time-resolved fluoroimmunoassays for HBsAg. One was Eu 3+ -BHHCT-HBsAb-TRFIA, another was Eu 3+ -BHHCT-BSA-SA-TRFIA. The detection limits of the two methods were 0.2ng/mL and 0.05ng/mL respectively. The standard curves for both methods were in the ranges of 0-100ng/mL and the within-run coefficient variations were less than 10%. The recovery rates of latter method were within 85%-115%. 118 patient samples were totally detected by Eu 3+ -BHHCT-BSA-SA-TRFIA and compared with a conventional ELISA, the results showed that the former could detect more positive samples than the latter.

关 键 词：铕荧光络合物 时间分辨荧光免疫分析法 乙型肝炎病毒表面抗原 定量测定 

分 类 号：R392[医药卫生—免疫学]