一种新的检测细胞凋亡的多参数流式细胞分析方法  

A New Method for Apoptosis Detection by Multi-parameter Flow Cytometry

作　　者：王珊[1] 梁学颖[1] 李宁[1] 周春喜[1] 于力方[1] 廖杰[1] 

机构地区：[1]解放军总医院医学实验测试中心,北京100853

出　　处：《标记免疫分析与临床》2002年第3期165-168,共4页Labeled Immunoassays and Clinical Medicine

摘　　要：建立了一种新的检测细胞凋亡的多参数流式细胞分析方法。HL6 0白血病细胞株经化疗药物足叶乙甙处理后 ,加AnnexinV (AV) -FITC/PI孵育双染 ,用多参数流式细胞术检测细胞凋亡。结果显示 ,凋亡细胞的百分比随诱导时间的延长而逐渐增多。表明AV -FITC/PT双染法既能对细胞膜表面特异蛋白染色 ,又同时检测细胞膜完整性。多参数流式细胞术是一种快速。To develop a method of analyzing apoptosis using multi-parameter flow cytometry, HL60 cell line was incubated with Annexin V-FITC/PI after exposure to etoposide, and the apoptosis of HL60 cell was monitored by flow cytometry. The result showed that the percentage of apoptosis cell increases with the time of exposure to etoposide. In conclusion, Annexin V FITC/PI method can not only detect special proteins, but also monitor the integrity of cell membrane. Multi-parameter flow cytometry is a rapid, easy and accurate method for the detection of apoptosis.

关 键 词：检测 流式细胞术 细胞凋亡 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]