Role of LOX3 Gene in Alleviating Adverse Effects of Drought and Pathogens in Rice  

作　　者：Nan-nan LIU Ling JIANG Wen-wei ZHANG Ling-long LIU Hu-qu ZHAI Jian-min WAN 

机构地区：[a] State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University/Jiangsu Plant Gene Engineering Research Center, Nanjing 210095, China [b] Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China

出　　处：《Rice science》2008年第4期276-282,共7页水稻科学（英文版）

基　　金：supported by the grants from the National Basic Research Program of China (Grant No. 2004CB117204 and No. 2006CB100200).

摘　　要：Lipoxygenase 3 (LOX3) is a major component of the LOX isozymes in mature rice seeds. To investigate the role of LOX3 gene under stresses, a plant expression vector containing antisense cDNA of LOX3 was constructed. Rice varieties Wuyunjing 7 and Kasalath were transformed by the Agrobacterium-mediated method and transgenic rice plants were generated. PCR and Southern blot results showed that the antisense LOX3 gene was integrated into the rice genome. Analyses of embryo LOX3 deletion and semi-quantitative RT-PCR confirmed the antisense suppression of LOX3 gene in transgenic plants. The T2 antisense plants of LOX3 were sensitive to drought stress, rice blast and bacterial blight compared with non-transgenic plants. These results suggest that the LOX3 gene might function in response to stresses.

关 键 词：RICE lipoxygenase gene antisense plant transgenic plants adverse stress drought stress rice blast bacterial blight gene function 

分 类 号：S511[农业科学—作物学]