Molecular Cytogenetic Analysis of Spontaneous Interspecific Hybrid Between Oryza sativa and Oryza minuta  

作　　者：Chuan-deng YI Xu CHENG Bei-bei WANG Guo-hua LIANG Zhi-yun GONG Shu-zhu TANG Ming-hong GU 

机构地区：[1]Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology/Key Laboratory of Plant Functional Genomics, Ministry of Education, Yangzhou University, Yangzhou 225009, China

出　　处：《Rice science》2008年第4期283-288,共6页水稻科学（英文版）

基　　金：supported by the National Natural Science Foundation of China (Grant No. 30500317 and No. 30170567).

摘　　要：Genomic in situ hybridization (GISH) is a powerful tool to characterize parental chromosomes in interspecific hybrids, including the behaviour of autosynapsis and chromosome pairing. It was used to distinguish the chromosomes of Oryza sativa from wild species in a spontaneous interspecific hybrid and to investigate the chromosome pairing at metaphase I in meiosis of the hybrid in this study. The hybrid was a triploid with 36 chromosomes according to the chromosome number investigated in mitosis of root tips. During metaphase I of meiosis in the hybrid, less chromosome pairing was observed and most of the chromosomes existed as univalent. Based on GISH and FISH (Fluorescent in situ hybridization) analyses, the chromosomes of the hybrid were composed of genomes A, B and C. Thus, it was believed that the hybrid was the result of natural hybridization between cultivated rice and wild species O. minuta which was planted in experimental fields.

关 键 词：wild rice interspecific hybrid genomic in situ hybridization fluorescent in situ hybridization molecular cytogenetics MEIOSIS chromosome pairing GENOME 

分 类 号：S511[农业科学—作物学]