核壳结构骨仿生纳米纤维的制备及生物性能研究  

The preparation and biological properties of core-shell structure bone biomimetic nanofibers

作　　者：邵伟力[1] 何建新[2] 陈利[1] 韩啟明 谭卫琳 崔世忠[1,2] 

机构地区：[1]天津工业大学纺织学院,天津300387 [2]中原工学院纺织学院,河南郑州450007

出　　处：《上海纺织科技》2016年第1期16-18 23,共4页Shanghai Textile Science & Technology

基　　金：国家自然科学基金项目(51203196);NSFC-河南人才培养联合基金(U1204510);河南省教育厅科学技术研究重点项目(14A540003;14A540006);郑州市普通科技攻关项目(141PPTGG400)

摘　　要：利用自制的同轴静电纺丝装置,以HA水溶液为核,TSF水溶液为壳,成功制备了不同比例的HA/TSF核壳结构纳米纤维。利用扫描电子显微镜(SEM)、透射电子显微镜(TEM)及拉伸测试对核壳结构纳米纤维的形貌和力学性能进行了研究。此外,还通过将不同核壳比HA/TSF的核壳结构纳米纤维在体外与人成骨肉瘤细胞(MG-63)进行复合培养,对其生物性能进行了研究,并通过荧光显微镜测试和四甲基偶氮噻唑蓝比色(MTT)法对核壳结构纳米纤维的生物学性能进行了表征。结果表明,核壳结构纳米纤维均具有良好的生物相容性,都能促进细胞的增殖,尤以HA/TSF为2∶1时效果最为明显。With TSF solution as the shell and HA water solution as the core,different core / shell ratio of HA / TSF nanofibers were successfully fabricated by a facile coaxial electrospinning technique. The morphology and mechanical properties of core-shell structure nanofibers were characterized by scanning electron microscopy( SEM), transmission electron microscopy( TEM) and tensile test.Furthermore,human osteosarcoma cells( MG- 63) was cultured in vitro to study the biological properties of core-shell structure nanofibers,which was characterized by fluorescence microscope test and four methyl azo thiazole blue colorimetric( MTT). The results indicated that the core-shell structure nanofibers showed good biocompatibility,which could promote cell adhesion,proliferation,especially in HA / TSF 2 ∶ 1group.

关 键 词：静电纺纳米纤维 核壳结构 羟基磷灰石 柞蚕丝素 生物性能 

分 类 号：TS102[轻工技术与工程—纺织工程]