冻存精子与卵子受精后行囊胚移植分娩一健康婴儿  

Birth of a healthy baby after transfer of blastocysts derived from cryopreserved human oocytes fertilized with frozen spermatozoa

作　　者：Tjer G.C.-C. Chiu T.T.-Y. Cheung L.-P. 朱国栋 

机构地区：[1]Dept. of Obstetrics and Gynecology, Prince of Wales Hospital, Shatin NT, Hong Kong Dr.

出　　处：《世界核心医学期刊文摘（妇产科学分册）》2005年第9期22-22,共1页Core Journal in Obstetrics/Gynecology

摘　　要：Objective: To report the birth of a healthy baby after transfer of blastocysts derived from frozen eggs and frozen spermatozoa. Design: Case report. Setting: University-based assisted reproduction center. Patient(s): A 37-year-old woma n with secondary infertility of 4 years’duration. Intervention(s): Retrieved oo cytes were cryopreserved in 1.5 M 1,2-propanediol (PROH) and 0.3 M sucrose by a slow freezing-rapid thawing protocol, semen cryopreservation, and insemination by intracytoplasmic sperm injection (ICSI) at 4 hours after thawing. Main Outco me Measure(s): Fertilization and embryo development to blastocyst stage, pregnan cy, and outcome. Result(s): Ten of 14 frozen oocytes survived after thawing. Eig ht of them were fertilized by performing ICSI and three developed into 7-to 8- cell embryos on day 3. Two of these embryos developed into blastocysts on day 5 and were transferred. This resulted in a successful pregnancy and the delivery o f a healthy baby boy. Conclusion(s): This case demonstrates the feasibility of i nseminating the frozen-thawed human oocytes after 4 hours of in vitro culture a nd the zygotes derived from frozen oocytes and frozen spermatozoa can be culture d to blastocysts resulting in the live birth of a healthy baby boy.Objective: To report the birth of a healthy baby after transfer of blastocysts derived from frozen eggs and frozen spermatozoa. Design: Case report. Setting: University-based assisted reproduction center. Patient(s): A 37-year-old woma n with secondary infertility of 4 years'duration. Intervention(s): Retrieved oo cytes were cryopreserved in 1.5 M 1,2-propanediol (PROH) and 0.3 M sucrose by a slow freezing-rapid thawing protocol, semen cryopreservation, and insemination by intracytoplasmic sperm injection (ICSI) at 4 hours after thawing. Main Outco me Measure(s): Fertilization and embryo development to blastocyst stage, pregnan cy, and outcome. Result(s): Ten of 14 frozen oocytes survived after thawing. Eig ht of them were fertilized by performing ICSI and three developed into 7-to 8- cell embryos on day 3. Two of these embryos developed into blastocysts on day 5 and were transferred. This resulted in a successful pregnancy and the delivery o f a healthy baby boy. Conclusion(s): This case demonstrates the feasibility of i nseminating the frozen-thawed human oocytes after 4 hours of in vitro culture a nd the zygotes derived from frozen oocytes and frozen spermatozoa can be culture d to blastocysts resulting in the live birth of a healthy baby boy.

关 键 词：囊胚移植 卵子受精 辅助生殖 卵母细胞 显微注射 囊胚期 继发性不孕 受精过程 妊娠过程 最终结局 

分 类 号：R714.8[医药卫生—妇产科学]