自体非培养黑色素细胞-角质细胞移植治疗节段型和局灶型白癜风的长期随访研究  

Long-term follow-up study of segmental and focal vitiligo treated by autologous, noncultured melanocyte-keratinocyte cell transplantation

作　　者：Mulekar S.V. 罗素菊 冯义国 

机构地区：[1]Natl. Ctr. Vitiligo and Psoriasis, Post Box 300320, Riyadh 11372, Saudi Arabia Dr.

出　　处：《世界核心医学期刊文摘（皮肤病学分册）》2005年第2期8-8,共1页Digest of the World Core Medical JOurnals:Dermatology

摘　　要：Objective: To evaluate long-term efficacy and safety of melanocyte-keratinocyte cell transplantation in the management of segmental and focal vitiligo. Design: A simpler and modified method based on that of Olsson and Juhlin was performed. This method uses a shaved biopsy skin sample up to one tenth the size of the recipient area. The skin sample is incubated, and the cells are mechanically separated using trypsin-EDTA solution and then centrifuged to prepare a suspension. Cell suspension is then applied to the dermabraded depigmented skin area, and a collagen dressing is applied to keep it in place. Patients: Fifty patients with segmental and 17 with focal vitiligo were treated. One patient with segmental and 2 with focal vitiligo did not attend any follow-up visits. The remaining patients were observed for a period of up to 5 years. Intervention: Autologous, noncultured melanocyte-keratinocyte cell transplantation. Main Outcome Measure: Repigmentation was graded as excellent with 95%to 100%pigmentation, good with 65%to 94%, fair with 25%to 64%, and poor with 0%to 24%of the treated area. Results: In the segmental vitiligo group, 41 patients (84%) showed excellent, 3 (6%) good, and 5 (10%) poor pigmentation, which was retained until the end of the respective follow-up period. In the focal vitiligo group, 11 patients (73%)-showed excellent, 1 (7%) fair, and 3 (20%) poor pigmentation, which was retained until the end of the respective follow-up period. Conclusions: Melanocyte-keratinocyte cell transplantation is a simple, safe, and effective surgical therapy. Patients with segmental and focal vitiligo can experience a prolonged disease-free period, which may extend through the rest of their lives.Objective: To evaluate long-term efficacy and safety of melanocyte-keratinocyte cell transplantation in the management of segmental and focal vitiligo. Design: A simpler and modified method based on that of Olsson and Juhlin was performed. This method uses a shaved biopsy skin sample up to one tenth the size of the recipient area. The skin sample is incubated, and the cells are mechanically separated using trypsin-EDTA solution and then centrifuged to prepare a suspension. Cell suspension is then applied to the dermabraded depigmented skin area, and a collagen dressing is applied to keep it in place. Patients: Fifty patients with segmental and 17 with focal vitiligo were treated. One patient with segmental and 2 with focal vitiligo did not attend any follow-up visits. The remaining patients were observed for a period of up to 5 years. Intervention: Autologous, noncultured melanocyte-keratinocyte cell transplantation. Main Outcome Measure: Repigmentation was graded as excellent with 95%to 100%pigmentation, good with 65%to 94%, fair with 25%to 64%, and poor with 0%to 24%of the treated area. Results: In the segmental vitiligo group, 41 patients (84%) showed excellent, 3 (6%) good, and 5 (10%) poor pigmentation, which was retained until the end of the respective follow-up period. In the focal vitiligo group, 11 patients (73%)-showed excellent, 1 (7%) fair, and 3 (20%) poor pigmentation, which was retained until the end of the respective follow-up period. Conclusions: Melanocyte-keratinocyte cell transplantation is a simple, safe, and effective surgical therapy. Patients with segmental and focal vitiligo can experience a prolonged disease-free period, which may extend through the rest of their lives.

关 键 词：节段型 黑色素细胞 局灶型 细胞移植治疗 随访研究 皮肤磨削 细胞悬液 皮肤标本 机械分离 受区 

分 类 号：R758.41[医药卫生—皮肤病学与性病学]