白介素19可上调角质形成细胞生长因子且与银屑病有关  

Interleukin- 19 upregulates keratinocyte gr- owth factor and is associated with psoriasis

作　　者：王琼 

出　　处：《世界核心医学期刊文摘（皮肤病学分册）》2005年第12期47-47,共1页Digest of the World Core Medical JOurnals:Dermatology

摘　　要：Background: Interleukin (IL)- 19, a member of the IL- 10 family, signals through the IL- 20R1/IL- 20R2 heterodimer, which is shown to be involved in abnormal keratinocyte differentiation and proliferation. Little is known about its in vitro biological functions or its role in psoriasis. Objectives: To investigate the role of IL- 19 in the psoriatic process. Methods: The expression of keratinocyte growth factor (KGF) transcripts was measured by polymerase chain reaction in CD8+ T cells treated with IL- 19. Next, we developed monoclonal and polyclonal antibodies to measure the levels of IL- 19 in the sera of patients with psoriasis and healthy volunteers using an enzyme- linked immunosorbent assay. In addition, we performed immunohistochemical staining on psoriatic skin and normal controls. Results: We found that IL- 19 upregulated KGF transcripts on CD8+ T cells. Patients with psoriasis had a lower level of IL- 19 in serum than healthy volunteers. The difference between these two groups was statistically significant (P < 0.05). IL- 19 expression was seen in basal and suprabasal keratinocytes in a continuous pattern, and was increased in psoriatic epidermis. Conclusions: These results suggest that IL- 19 plays a role in the complex pathological cytokine network in psoriasis.Background: Interleukin (IL)- 19, a member of the IL- 10 family, signals through the IL- 20R1/IL- 20R2 heterodimer, which is shown to be involved in abnormal keratinocyte differentiation and proliferation. Little is known about its in vitro biological functions or its role in psoriasis. Objectives: To investigate the role of IL- 19 in the psoriatic process. Methods: The expression of keratinocyte growth factor (KGF) transcripts was measured by polymerase chain reaction in CD8+ T cells treated with IL- 19. Next, we developed monoclonal and polyclonal antibodies to measure the levels of IL- 19 in the sera of patients with psoriasis and healthy volunteers using an enzyme- linked immunosorbent assay. In addition, we performed immunohistochemical staining on psoriatic skin and normal controls. Results: We found that IL- 19 upregulated KGF transcripts on CD8+ T cells. Patients with psoriasis had a lower level of IL- 19 in serum than healthy volunteers. The difference between these two groups was statistically significant (P < 0.05). IL- 19 expression was seen in basal and suprabasal keratinocytes in a continuous pattern, and was increased in psoriatic epidermis. Conclusions: These results suggest that IL- 19 plays a role in the complex pathological cytokine network in psoriasis.

关 键 词：银屑病 角质形成细胞 基底层 二聚体 信号物质 显著性差异 中起 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]