外源一氧化氮对大鼠胰岛细胞损伤机制研究  被引量：14

Mechanism of destruction of islet cells by exogenous nitric oxide</em> <p class='article-op'> <span id="bdshare" class="bdshare_t bds_tools get-codes-bdshare baidufx"><span><span class="bds_more" style="display: block; width: 62px; height: 29px; background: url(/images/share.gif) no-repeat!important; padding: 0;"></span></span></span> </p> <p class="article-source"> <a href ='/article/read.aspx?id=6837855' class='btnTitle btnIsView' target='_blank'><img alt='在线阅读' src='/images/btn-read.png'/></a><a class='btnTitle btnIsView' href="javascript:showdown('MgdFDkiTxz47W2W5iGhfKS2ONasB/x2X7eGYmuPLRko=')"><img alt="下载全文" src="/images/btn-down.png" /></a> <input id="hiddowninfo" type="hidden" value="MgdFDkiTxz47W2W5iGhfKS2ONasB/x2X7eGYmuPLRko="/> </p> <div class="collect" style="display: none"> <h5>我的收藏</h5> <div class="cnt"> <p> 名 称：<input type="text" name="collTitle" id="collSumTitle" /> </p> <p> 注 释：<input type="text" name="collRemark" id="collSumRemark" /> </p> <p style="text-align: center;"> <a href="javascript:void(0)" onclick="g_collectSumOk();"> <img width="65" height="17" src="/images_new/collect_ok.png" alt="完成" /></a> <a href="javascript:void(0)" onclick="g_collectSumCancel();"> <img width="65" height="17" src="/images_new/collect_no.png" alt="取消" /></a> </p> </div> </div> </div> <form name="wxTransfer" method="post"> <input type="hidden" name="lid" value="" /> <input type="hidden" name="sip" value="" /> <input type="hidden" name="date" value="" /> <input type="hidden" name="co" value="" /> <input type="hidden" name="Title_C" value="" /> <input type="hidden" name="Writers" value="" /> <input type="hidden" name="Name_C" value="" /> <input type="hidden" name="Num" value="" /> <input type="hidden" name="Years" value="" /> <input type="hidden" name="Volumn" value="" /> <input type="hidden" name="GCH" value="" /> <input type="hidden" name="Vol" value="" /> <input type="hidden" name="BookID" value="" /> <input type="hidden" name="SubjectNum" value="" /> <input type="hidden" name="SpecialNum" value="" /> <input type="hidden" name="mirrorName" value="" /> <input type="hidden" name="UserID" value="" /> <input type="hidden" name="UserName" value="" /> <input type="hidden" name="sourceID" value="" /> </form> <script type="text/javascript" src="/scripts/transfer.min.js"></script> <div class="article-detail"> <p class='author'><strong>作　　者：</strong><a href='/writer/article.aspx?id=100000034746491' target='_blank' ><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=A%3d%5b%e6%9d%8e%e5%bc%ba%5d' title='李强[1]'>李强[1]</a> <a href='/writer/article.aspx?id=100000007836148' target='_blank' ><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=A%3d%5b%e8%ae%b8%e9%9c%96%e6%b0%b4%5d' title='许霖水[2]'>许霖水[2]</a> <em> </em></p><p class='organ'><strong>机构地区：</strong><a href='/organ/article.aspx?id=487&subid=&showname=&searchtype=' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=S%3d%e7%ac%ac%e4%b8%89%e5%86%9b%e5%8c%bb%e5%a4%a7%e5%ad%a6%e6%96%b0%e6%a1%a5%e5%8c%bb%e9%99%a2%e6%a3%80%e9%aa%8c%e7%a7%91' title='[1]第三军医大学新桥医院检验科,重庆400038'>[1]第三军医大学新桥医院检验科,重庆400038</a> <a href='/organ/article.aspx?id=295&subid=&showname=&searchtype=' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=S%3d%e7%ac%ac%e4%b8%89%e5%86%9b%e5%8c%bb%e5%a4%a7%e5%ad%a6%e7%94%9f%e5%8c%96%e6%95%99%e7%a0%94%e5%ae%a4' title='[2]第三军医大学生化教研室,重庆400038'>[2]第三军医大学生化教研室,重庆400038</a> <em> </em></p><p class='from'><strong>出　　处：</strong><a href='/journal/summary.aspx?gch=91335X' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=J%3d%5b%e8%a5%bf%e5%8c%97%e5%9b%bd%e9%98%b2%e5%8c%bb%e5%ad%a6%e6%9d%82%e5%bf%97%5d' title='西北国防医学杂志'>《西北国防医学杂志》</a>2002年第5期361-363,共3页<em>Medical Journal of National Defending Forces in Northwest China</em></p><p class='fund'><strong>基　　金：</strong>.NULL.</p><p class='abstrack'><strong>摘　　要：</strong>目的:研究外源NO对胰岛细胞的损伤机制和功能的影响。方法:采用DNA电泳技术以及单细胞电泳检测NO对胰岛细胞的凋亡和DNA的损伤作用;通过测定胰岛素含量检测胰岛细胞在NO作用下的合成和分泌功能。结果:外源NO能导致胰岛细胞DNA的断裂形成彗星细胞,DNA电泳显示典型梯状条带,大剂量NO形成死亡条带;同时NO能抑制胰岛细胞合成和分泌胰岛素。结论:外源NO可导致胰岛细胞凋亡、DNA断裂损伤并抑制其合成分泌胰岛素的功能。<em><Abstrcat>Objective:To study the mechanism of the destruction and changes of function of islet cells caused by exogenous nitric oxide(NO).Methods:The damage of DNA and apoptosis in islet cells were assessed in vitro with single cell gel electrophoresis and gel electrophoresis of DNA. Insulin synthesis and secretion of normal rats pancreas under high level ofglucose caused by NO were detected.Results:The exogenous NO caused breakage of DNA strand .The islet cells showed remarkable apoptotic ladder with low dose of NO and necrosis ladder with high dose.The insulin synthesis and secretion of islet cells under high level of glucose were inhibited by NO in vitro.Conclusion:The exogenous NO can directly induce islet cells apoptosis,DNA breaking and inhibit their function.</em></p><p class='subject'><strong>关 键 词：</strong><a href='/subject/article.aspx?id=965701' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=K%3d%5b%e8%83%b0%e5%b2%9b%e7%bb%86%e8%83%9e%5d' title='胰岛细胞'>胰岛细胞</a> <a href='/subject/article.aspx?id=922414' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=K%3d%5b%e4%b8%80%e6%b0%a7%e5%8c%96%e6%b0%ae%5d' title='一氧化氮'>一氧化氮</a> <a href='/subject/article.aspx?id=4085951' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=K%3d%5b%e7%bb%86%e8%83%9e%e5%87%8b%e4%ba%a1%5d' title='细胞凋亡'>细胞凋亡</a> <a href='/subject/article.aspx?id=965545' target='_blank'><b class='type_ico_id'></b></a><a href='/zk/search.aspx?key=K%3d%5b%e8%83%b0%e5%b2%9b%e7%b4%a0%e4%be%9d%e8%b5%96%e5%9e%8b%e7%b3%96%e5%b0%bf%e7%97%85%5d' title='胰岛素依赖型糖尿病'>胰岛素依赖型糖尿病</a> </p><p class='class'><strong>分 类 号：</strong><a href='/zk/search.aspx?key=C%3dR587.1' title='R587.1'>R587.1</a>[<a href = '/domain/article.aspx?id=494' >医药卫生—内分泌</a>] </p> </div> <div id="content_wzxg" name="wzxg" class="article-graph"> <object width="695" height="250" classid="clsid:D27CDB6E-AE6D-11cf-96B8-444553540000" id="FlashID"> <param value="../flash/relation.swf" name="movie"> <param value="high" name="quality"> <param value="opaque" name="wmode"> <param value="6.0.65.0" name="swfversion"> <param value="dataUrl=/ajax/articlerelativegraph.ashx?id=6837855" name="flashVars"> <embed width="695" height="250" 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