登革病毒组织培养技术  

The tissue culture techniques of Dengue virus

作　　者：李雪莲[1] 武峰[1] 王莹[1] 

机构地区：[1]郑州大学基础医学院病原生物教研室,河南郑州450052

出　　处：《河南医学研究》2002年第3期217-219,共3页Henan Medical Research

摘　　要：目的 :探讨对登革病毒敏感性不同的几株蚊子细胞和哺乳动物细胞的体外培养技术。方法 :对白纹伊蚊C6/3 6细胞、伪盾伊蚊Ap 61细胞和三株哺乳动物细胞LLC MK2 细胞、Vero细胞、BHK 2 1/3 1细胞进行体外培养 ,把登革病毒转染到上述细胞中 ,经传代培养后 ,在显微镜下观察。结果 :通过观察结果及时改进各细胞株的培养基的配制、消化方法和传代比例 ,各细胞株均能生长良好、单层致密、无污染 ,登革病毒转染实验呈阳性结果。结论 :实验结果证明白纹伊蚊C6/3 6细胞、Ap 61细胞、LLC MK2 细胞、Vero细胞、BHK 2 1/3 1细胞均为登革病毒敏感细胞 ,其中白纹伊蚊C6/3 6细胞敏感性较高 ,随着新的克隆细胞株的体外培养技术的成熟 ,组织培养已成为登革病毒分离。Objective: To explore the sensitivity of cells to Dengue virus,we will introduce the techologies by which several kinds of cells were cultured.Methods: The cells include Aedes albopictus C6/36 cell,Ap 61 cell,LLC MK 2 cell,Vero cell,DHK 21/31 cell were cultived external body and influenced by Dengue virus,we can observe the cells under the microscope.Results: Though observed the cells we could improve the cultural media and digest method and the genetic percentage in time.All cells could express well and without pullution,the results of Dengue virus infect test were positive.Conclusion: The experiments proved upside cells all sensitive to the Dengue virues and the Aedes albopictus C6/36 cell is the best.With the development of new colony cells culture,the tissue culture techniques of Dengue virus become more and more important

关 键 词：登革病毒 组织培养技术 C6/36细胞 Ap-61细胞 LLC-MK2细胞 VERO细胞 BHK-21/31细胞 

分 类 号：R373.33[医药卫生—病原生物学]