水稻齿叶矮缩病毒S8基因编码的结构蛋白质及其自聚集性质  

Self aggregation of the Structural Protein Encoded by Rice Ragged Stunt Oryzavirus Genome Segment 8

作　　者：吕慧涓[1] 邵朝纲[1] 龚祖埙[1] 

机构地区：[1]中国科学院上海生命科学研究院生物化学与细胞生物学研究所,蛋白质组学重点实验室,上海200031

出　　处：《生物化学与生物物理学报》2002年第5期565-570,共6页

基　　金：国家自然科学基金资助项目 (No .30 1 70 0 42 )

摘　　要：水稻齿叶矮缩病毒是一种以水稻为侵害寄主的植物呼肠孤病毒 ,其基因组 1 0条dsRNA编码的蛋白质产物的功能除S9外的大部分还未阐明。报道了该病毒菲律宾分离株S8的开放阅读框序列 ,在大肠杆菌中表达和纯化出了其 6 7kD的蛋白质产物 ,并进一步研究了该产物的功能。实验结果表明 ,S8的蛋白质产物是病毒的一种主要结构蛋白质 ,在体外具有自剪切活性和自聚集性质 。Rice ragged stunt oryzavirus (RRSV) is a member of the genus oryzavirus within the family Reoviridae. Its genome consists of ten segments of dsRNA. The functions of all products encoded by these viral genome segments, except one encoded by S9, have not yet been elucidated. In the present study, the ORF of S8 of RRSV Philippines isolate was sequenced and expressed in E. coli . The 67 kD product of S8 could be self cleaved into two fragments with molecular weights of 43 kD and 26 kD. Western blotting indicated that both 67 kD and 43 kD products were major structural proteins of the virus. It was also found that the 67 kD protein could self aggregate into aggregates with higher sedimentation rate in sucrose gradients during centrifugation. Moreover, the self aggregation process could be accelerated by the complex of S6 product and genome dsRNAs of RRSV. These results suggest that the S8 products, 67 kD or 43 kD, may be the structural components of the viral inner capsid.

关 键 词：水稻 齿叶矮缩病毒 S8基因编码 结构蛋白质 自聚集性质 

分 类 号：S435.111[农业科学—农业昆虫与害虫防治]