清开颗粒对急性肝坏死大鼠肝脏的组织学影响  

Influence of "Qing Kai Granules" on Morphology of Rat Liver with Acute Liver Necrosis

作　　者：卓蕴慧[1] 陈建杰[1] 王灵台[1] 

机构地区：[1]上海中医药大学附属曙光医院,上海200021

出　　处：《上海中医药大学学报》2002年第3期54-56,共3页Academic Journal of Shanghai University of Traditional Chinese Medicine

基　　金：上海市卫生局资助项目 (98L0 0 8)

摘　　要：为探讨清开颗粒 (主药 :生大黄、败酱草、赤芍药、石菖蒲 )抗急性肝坏死的作用机制。分别以D GalN加LPS染毒和TAA染毒Wistar大鼠造成两种急性肝坏死动物模型 ,并分别分为模型组 ,清开颗粒预防 (造模前后两次给药 )、治疗组 (造模后即给药 )和HGF对照组 (治疗组 ,预防组 ) ,48h后处死动物 ,观察肝组织病理变化 (HE染色、网状纤维染色 )和PCNA免疫组化等指标。结果 :清开颗粒治疗组和预防组的病理改变明显轻于模型组和HGF对照组。提示 :清开颗粒有减轻肝脏炎性程度 。In order to explore the functional mechanism of 'Qing Kai Granules' (main ingredients:Radix Rhei,Herba Patriniae,Radix Paeoniae Rubra,Rhizoma Acori Graminei) in resisting acute liver necrosis,D GalN plus LPS poisoned and TAA poisoned Wistar rats were made into two types of animal models with acute liver necrosis and were divided into the model group,'Qing Kai Granules' prevention group (administrated twice before and after model),treatment group (administrated immediately after model) and HGF control group.The animals were killed 48 hours later,to observe the indexes related to the pathological change of the liver tissues (HE staining,reticular fibers staining) and PCNA immunohistochemistry.The results showed that the pathological change was remarkably slighter in the treatment group and prevention group of 'Qing Kai Granules' than in the model group and HGF control group,indicating that 'Qing Kai Granules' can reduce inflammatory degree of the liver and has a certain function to promote the re growth of the liver cells.

关 键 词：急性肝坏死 清开颗粒 免疫组化 动物模型 组织学 

分 类 号：R285.5[医药卫生—中药学]