紫秋刺葡萄离体繁殖研究  

Research on the Vitro Propagation of Purpl-Fall Spurs Grape

作　　者：周恒[1] 万召娣 罗静[1] 

机构地区：[1]铜仁职业技术学院生物工程系 [2]铜仁市川硐镇农技站

出　　处：《铜仁职业技术学院学报》2011年第2期30-33,共4页Academic forum of Tongren Polytechnic College

摘　　要：以紫秋刺葡萄单芽茎段为试材,研究消毒剂类型及时间、激素组合、培养基类型等对紫秋刺葡萄离体繁殖的影响。结果表明:在半无菌处理的前提下,以0.1%的升汞处理3分钟消毒效果最佳;最合适的芽诱导培养基是1/2MS+BA1.5mg/l+IBA0.1mg/l,芽增殖培养基是MS+BA1.0mg/l+IBA0.5mg/l,生根培养基是1/2MS+NAA0.5mg/l。As the test material,the single-bud stem section of purpl-fall spurs grape is used to study the effect on the disinfectant type,time,hormone combinations,media type,etc.to the vitro propagation of purpl-fall spurs grape.The results show that the best effect is on the contion of the premise of half-sterile and 0.1% mercuric chloride for 3 min disinfection;the most suitable bud induction medium was 1/2MS + BA1.5mg/l + IBA0.1mg/l,multiplication medium was MS + BA1.0mg/l + IBA0.5mg/l,rooting medium is 1/2MS + NAA0.5mg/l.

关 键 词：刺葡萄 离体繁殖 激素组合 培养基 

分 类 号：S663.1[农业科学—果树学]