酸性铬深蓝共振光散射光谱法测定蛋白质的研究  

Study on Determination of Protein with Acid Chrome Dark Blue by Resonance Light-scattering Method

作　　者：李树伟[1] 王照丽[1] 钱一鸣[1] 张姝[1] 陈华芳[1] 易鸣[2] 

机构地区：[1]四川师范大学化学与生命科学学院,四川成都610066 [2]四川师范大学校医院,四川成都610066

出　　处：《四川师范大学学报（自然科学版）》2002年第5期494-496,共3页Journal of Sichuan Normal University（Natural Science）

摘　　要：基于人血清白蛋白 (HSA)对酸性铬深蓝共振光散射的增强效应 ,拟定了一种新的测定HSA的共振光散射法 .在pH =3.78的B R缓冲溶液中 ,酸性铬深蓝与蛋白质结合 ,产生强烈的共振光散射 ,在λ =36 5nm处 ,共振光散射强度较大 ,且共振光散射强度与蛋白质的浓度成线性关系 ,线性范围为 0 .0～ 12 .0 μg mL ,检测限可达 0 .13μg mL .该法简便、快速 ,用于人体血清样品蛋白质的测定 ,结果令人满意 .In this paper, the reaction between acid chrome dark blue and HSA is studied. In the Britton Robinson buffer at pH=3.78, acid chrome dark blue combines with protein by intermolecular forces, which results in a resonance light scattering (RLS). The RLS intensity reaches maximum at 365 nm and is proportional to the concentration of protein. The assay is simple, rapid and sensitive with a linear range up to 12.0 μg/mL and a detection limit at 0.13 μg/mL. This assay is applied to the determination of human serum protein, and the results are satisfactory.

关 键 词：酸性铬深蓝 共振光散射光谱法 蛋白质 含量测定 人血清白蛋白 吸收光谱 

分 类 号：Q512.103[生物学—生物化学]