补骨脂中两个黄酮类成分的高效液相色谱测定  

HPLC Determination of Two Flavonoid Compounds in Psoralea corylifolia

作　　者：林瑞民[1] 王大为[2] 熊志立[2] 许勇[2] 李发美[2] 

机构地区：[1]鞍山师范学院化学系,辽宁鞍山114005 [2]沈阳药科大学药学院,辽宁沈阳110016

出　　处：《中国中药杂志》2002年第9期669-671,共3页China Journal of Chinese Materia Medica

摘　　要：目的 :同时测定补骨脂中的两个黄酮类成分。方法 :以实验室制备的补骨脂二氢黄酮和补骨脂异黄酮为对照品 ,采用反相高效液相色谱法测定含量。TechsphereODS色谱柱 (4.6mm× 2 5 0mm ,5 μm) ;流动相为甲醇 2 0mmol·L-1醋酸铵缓冲液 (pH 4 .0 ) (6 7∶33) ;检测波长 2 4 0nm。 结果 :补骨脂二氢黄酮和补骨脂异黄酮浓度为 1.2 5～ 2 0 μg·mL-1,线性关系良好 ;补骨脂二氢黄酮和补骨脂异黄酮的平均回收率分别为 94 .9%和 96 .2 % ;RSD分别为 3.1%和 3.6 %。结论 :首次建立了同时测定补骨脂中补骨脂二氢黄酮和补骨脂异黄酮的HPLC分析方法 ,本方法结果准确 ,重现性好 ,适用于补骨脂黄酮类成分的分析测定。Objective: To determine two flavonoid compounds in Psoralea corylifolia L. (PC) simultaneously with HPLC method. Method: Bavachin and corylin isolated from PC and purified in our laboratory were used as the reference compounds. The HPLC separation was carried out on an Techsphere ODS column using mobile phase consisting of a mixture of methanol and 20 mmol·L -1 ammonium acetate buffer pH 4.0 (67∶33), and the UV detection wavelength was 240 nm. Result: Simultaneous determination of bavachin and corylin was achieved. The linear range was 1.25～20 μg·mL -1 for both bavachin and corylin. The average recovery of bavachin and corylin was 94.9% and 96.2%, and RSD was 3.1% and 3.6% respectively. Conclusion: This is the first report on simultaneous determination of bavachin and corylin in PC with satisfactory accuracy and reproducibility.

关 键 词：补骨脂 补骨脂二氢黄酮 补骨脂异黄酮 RP-HPLC法 含量测定 

分 类 号：R284[医药卫生—中药学]