白芷总挥发油对疼痛模型大鼠的β-内啡肽、促肾上腺皮质激素、一氧化氮及前阿黑皮素的影响  

Effect of Essential Oil of Radix Angelicae Dahuricae on β-endorphin,ACTH,NO and Proopiomelanocortin of Pain Model Rats

作　　者：聂红[1] 沈映君[2] 

机构地区：[1]南京中医药大学,江苏南京210029 [2]成都中医药大学,四川成都610075

出　　处：《中国中药杂志》2002年第9期690-693,共4页China Journal of Chinese Materia Medica

摘　　要：目的 :探讨白芷总挥发油 (essentialoilofRadixAngelicaeDahuricae ,EOAD)对甲醛所致的伤害性疼痛模型大鼠的β 内啡肽、促肾上腺皮质激素 (ACTH)、一氧化氮 (NO)及前阿黑皮素 (Proopiomelanocortin ,POMC)的影响。方法 :分别采用放射免疫法测定 β 内啡肽、ACTH ,化学法测定NO的含量 ;并用原位杂交方法研究EOAD对前阿黑皮素信使核糖核酸 (POMCmRNA)基因表达的影响。结果 :EOAD能显著升高甲醛所致的伤害性疼痛模型大鼠下丘脑的 β 内啡肽、NO含量 ;使下丘脑、脑干POMCmRNA表达阳性细胞数大幅度增加 ;而对血中的ACTH没有影响。结论 :EOAD可能通过增加中枢POMCmRNA表达阳性细胞数和内源性镇痛物质含量 ,激活内源性镇痛机制而镇痛 ,其作用部位可能在中枢。Objective:To investigate the effect of essential oil of Radix Angelicae Dahuricae (EOAD) on β endorphin?Adrenocorticotropic hormone(ACTH)?Nitric oxide(NO) and Proopiomelanocortin (POMC) of pain model rats induced by formaldehyde. Method:β endorphin and ACTH were detected with radio immunity, the amount of masculine cell expression of Proopiomelanocortin mRNA in hypothalamus, and brainstem of pain model was detected with in situ hybridization and NO with chemical method. Result:EOAD could increase β endorphin, NO and the amount of masculine cell expression of Proopiomelanocortin mRNA significantly in hypothalamus and brainstem of rats. Conclusion:EOAD which may be central analgesic, is associated with endogenous opiate like substance such as POMC and β endorphin.

关 键 词：白芷总挥发油 疼痛模型 促肾上腺皮质激素 一氧化氮 前阿黑皮素 放射免疫法 Β-内啡肽 

分 类 号：R285.5[医药卫生—中药学]