丹参酸乙对大鼠心脏成纤维细胞增殖和胶原基因表达的影响  

Effects of salvianolic acid B on the proliferation of cardiac fibroblasts and their expression of collagens genes

作　　者：童普德[1] 吴小江[1] 王晓玲[1] 

机构地区：[1]上海中医药大学生物学教研室,上海200032

出　　处：《中药药理与临床》2002年第4期7-9,共3页Pharmacology and Clinics of Chinese Materia Medica

基　　金：上海中医药大学科研基金资助

摘　　要：目的 :探讨丹参酸乙 (SA B)对血管紧张素Ⅱ (AngⅡ )孵育的新生大鼠心脏成纤维细胞功能的影响。 方法 :将培养的心脏成纤维细胞分成四组 :空白对照组 ;AngⅡ组 :培养基中加了 10 7mol/LAngⅡ ;SA B10 6mol/L组 :培养基中加 10 7mol/LAngⅡ+ 10 6mol/LSA B ;SA B10 4mol/L组 :培养基中加 10 7mol/LAngⅡ + 10 4mol/LSA B。加药后 2 4h分别作3 H TdR细胞增殖测定和采用逆转录多聚酶链反应 (RT PCR)测定Ⅰ、Ⅲ型胶原基因的转录水平。结果 :10 4mol/LSA B可有效抑制由AngⅡ引起的心脏成纤维细胞的增殖。 10 5mol/LSA B可抑制胶原Ⅲ型mRNA的表达量 ,而 10 6mol/LSA B和 10 5mol/LSA B都可显著抑制胶原Ⅰ型mRNA的表达。结论 :丹参酸乙可逆转由AngⅡ引起的心脏成纤维细胞的增殖和Ⅰ、Ⅲ型胶原基因的表达水平。Objective: To investigate how salvianolic acid B (SA B) affected function of cardiac fibroblasts incubated with angiotensin Ⅱ (AngⅡ). Methods: neonatal cardiac fibroblast were randomized into 4 groups: control group; AngⅡ group (added 10 7 mol/L AngⅡ in culture medium); SA B group (added 10 7 mol/L AngⅡ and two different concentration of SA B in culture medium). 24h after administration, the proliferation of cardiac fibroblasts was determined by cpm of 3H TdR, and the collagen Ⅰ and Ⅲ type mRNA expression were detected by RT PCR. Results: 10 7 mol/L AngⅡ resulted in a signifincant proliferation of cardiac fibroblasts, which could be inhibited by 10 4 mol/L SA B; and in a signifincant increase of the collagen Ⅰ and Ⅲ type mRNA expression, which could be inhibited by 10 5 mol/L SA B. Conclusion: 10 4 mol/L and 10 5 mol/L SA B could respectively reverse the proliferation and the collagen Ⅰ and Ⅲ type mRNA expression of cardiac fibroblasts induced by AngⅡ.

关 键 词：细胞增殖 胶原基因 丹参酸乙 血管紧张素Ⅱ 心脏成纤维细胞 RT-PCR 心肌纤维化 药理作用 

分 类 号：R285.5[医药卫生—中药学] R286.7[医药卫生—中医学]