Lazaroid U-74389G预处理对大鼠心脏停搏供体肝移植缺血再灌注损伤的保护作用  

Protection of Lazaroid U-74389G pretreatment against graft warm ischemia reperfusion injury in rat liver transplantation from non-heart-beating donors

作　　者：严佶祺[1] 李宏为[1] 张明钧[1] 杨卫平[1] 蔡伟耀[1] 

机构地区：[1]上海第二医科大学附属瑞金医院外科,200025

出　　处：《中华普通外科杂志》2002年第9期533-535,共3页Chinese Journal of General Surgery

基　　金：上海市科学技术发展基金资助项目 (964 1190 2 7)

摘　　要：目的探讨在心脏停搏供体肝移植中 ,LazaroidU 74 389G预处理对大鼠供肝热缺血损伤的保护作用。方法实验以U 74 389G预处理与否、以及供肝获取前经历的心脏停搏时间 4 5或6 0min分为 4组 ,分别为N 4 5组、N 6 0组、tN 4 5组和tN 6 0组 ,而后行大鼠原位肝移植 ,比较各组术后存活率、肝功能、MDA和肝脏病理学改变。结果N 4 5组、N 6 0组、tN 4 5组和tN 6 0组肝移植术后1周的存活率分别为 2 5 % (2 / 8)、0 (0 / 8)、5 8% (7/ 12 )和 33% (4/ 12 )。U 74 389G的预处理可以显著提高心脏停搏供体肝移植的存活率 ,并能够改善术后肝功能和减轻肝脏病理学改变 ,降低MDA的表达。结论在心脏停搏供体肝移植中 ,U 74 389G预处理可以减轻热缺血再灌注对供肝造成的损伤 。ObjectiveTo investigate the protective role of Lazaroid U 74389G pretreatment against graft warm ischemia reperfusion injury of rat liver transplantation from non heart beating donors.MethodsRat liver transplantation was carried out in 4 groups (N 45, N 60, tN 45 and tN 60), according to pretreatment with U 74389 G or not, and the non heart beating time of 45 or 60 minutes before donor liver was harvested.Survival rates, liver functions, MDA values and graft pathologies were compared between the groups.ResultsThe one week survival rates of group N 45, N 60, tN 45 and tN 60 were 25%(2/8), 0(0/8), 58%(7/12) and 33%(4/12) respectively.U 74389G pretreatment not only significantly increased survival rate of rat liver transplantation from non heart beating donors, but also improved liver functions and graft pathologies, as well as decreased MDA expression.Conclusions U 74389G pretreatment could attenuate ischemia reperfusion injury on donor livers and increase the survival rates in rat liver transplantation from non heart beating donors.

关 键 词：保护作用 肝移植 再灌注损伤 移植预处理 

分 类 号：R657.3[医药卫生—外科学]