定点突变对BPI_(23)-Fcγ1重组抗菌蛋白表达和复性率的影响  

Effects on the Expression and Renaturation of BPI_(23)-Fcγ1 Recombinant Bactericidal Protein by Site-directed Mutagenesis

作　　者：安云庆[1] 柯岩[1] 靖学芳[2] 杨贵贞 

机构地区：[1]首都医科大学免疫学系 [2]吉林大学基础医学院免疫学系

出　　处：《首都医科大学学报》2002年第3期197-201,共5页Journal of Capital Medical University

基　　金：国家自然科学基金资助项目 ( 395 70 6 6 6 )

摘　　要：为提高BPI2 3 Fcγ1重组抗菌蛋白在原核表达系统中的表达和复性率 ,采用定点突变法改造 pBV BPI60 0 Fcγ1 70 0 重组表达载体 ,转化E .coliDH5α后 ,通过温控诱导表达。结果表明 :①突变后BPI2 3 Fcγ1重组抗菌蛋白表达量比突变前提高约 1 0 % ;②表达时间提前约 1h ;③抗菌活性未受影响 ;In order to improve the expression and renaturation yield of BPI 23-Fcγ1 bactericidal recombinant protein in Prokaryotic system, pBV-BPI 600-Fcγ1 700 recombinant expression vector was reconstructed by site-directed mutation method. The mutational vector was transformed into the competent Escherichia coli DH5α and expressed by temperature induced method. The results showed the amount of the mutational BPI 23-Fcγ1 protein expression was 10% more than that of the non-mutant one, and its expression time was an hour earlier than the latter. Its bactericidal capability was the same as before, but its renaturation yield was not improved observably.

关 键 词：定点突变 pBV-BPI600-Fcγ1700重组表达载体 BPI23-Fcγ1重组抗菌蛋白 

分 类 号：R392.1[医药卫生—免疫学]