脐血CD34^+细胞体外短期培养扩增研究  

Study on Cord Blood CD34^+ Cell in Short-term Culture in Vitro Amplification

作　　者：苏力[1] 田丁[1] 万岁桂[1] 刘聪艳[1] 徐娟[1] 

机构地区：[1]首都医科大学宣武医院血液科

出　　处：《首都医科大学学报》2002年第3期218-221,共4页Journal of Capital Medical University

基　　金：北京市卫生局重点扶持学科基金资助课题

摘　　要：为寻找更有效的体外扩增脐血CD34 + 细胞的造血细胞因子组合 ,采集健康产妇脐带血 ,用免疫磁珠法分选CD34 + 细胞。采用SCF、FLT3 L、TPO和IL 34种具有早期作用的细胞因子的不同组合进行脐血CD34 + 细胞短期无血清液体培养 ,观察培养前后有核细胞、CD34 + 细胞、CD34 + /CD38- 细胞、CFU GEMM、CFU GM和BFU E数量的变化。结果在 3种不同的细胞因子组合中 ,同时应用SCF、FLT3 L、TPO和IL 34种细胞因子培养 7d的扩增效果最好。突出的发现是在这种条件下CD34 + /CD38- 细胞亚群达到平均 1 97.9倍的扩增效果。提示 :SCF、FLT3 L、TPO和IL 34种细胞因子是脐血CD34 +The objective was to explore the optimal cytokine combinations for in vitro amplification of the cord blood CD34 + cell. We collected the cord blood from normal full-term deliveries, and isolated CD34 + cells using mini MACS laboratory separation system. The purified CD34 +cells were cultured in static short-term serum free culture system with different combinations of SCF, FLT3-L, TPO and IL-3. Result: Within the three different cytokine combinations, the combination of SCF, FLT3-L, TPO and IL-3 was proved to be the most effective, and the notable result was that the amplification of CD34 +/CD38 - subsets reached 197.9 folds. Conclusion: The SCF, FLT3-L, TPO and IL-3 combination is the optimal cytokine combination for in vitro cord blood CD34 + cell amplification.

关 键 词：脐血CD34^+细胞 造血生长因子 CD34^+细胞体外扩增 CD34^+/CD38^+细胞亚群 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]