胶原膜、聚乳酸聚乙醇酸膜和聚β羟基丁酯的生物相容性  

Biodegradation and biocompatibility of collagen membrane, poly (lactic acid/glycolic acid) and poly-3-hydroxybutyrate

作　　者：赵东锷[1] 刘维永[1] 汪钢[1] 俞世强[1] 

机构地区：[1]第四军医大学西京医院心血管外科,陕西西安710033

出　　处：《第四军医大学学报》2002年第18期1694-1697,共4页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金资助项目 ( 39970 733)

摘　　要：目的　评价胶原膜、聚乳酸聚乙醇酸膜和聚 β羟基丁酯的结构特点、生物相容性及其在组织工程心脏瓣膜中的应用前景 .方法　 HE、胶原染色及扫描电镜观察材料的组成、结构 .新西兰大白兔 15只皮下植入材料 ,于 4,6 ,8,12 wk取出观察其组织反应和降解情况 .结果　胶原膜、聚乳酸聚乙醇酸膜和聚 β羟基丁酯的孔径分别为 10 7,179和 13μm. 3种材料组织相容性较好 ,局部无组织坏死和脓肿形成 .早期以中性粒细胞和嗜酸性粒细胞浸润为主 ,后期以淋巴细胞浸润为主 .聚乳酸聚乙醇酸膜于 8wk基本吸收 ,胶原膜 12 wk吸收 ,聚β羟基丁酯膜 12 wk未见明显降解吸收 .结论　胶原膜和聚乳酸聚乙醇酸膜在材料结构、组织相容性和降解率方面符合组织工程心脏瓣膜支架材料的要求 ,聚β羟基丁酯膜在材料结构和降解率方面尚需改进 .AIM To evaluate the constructive characteristics,biodegradation and biocompatibility of collagen membrane, poly (lactic acid/glycolic acid) and poly-3-hydroxybutyrate (PHB). METHODS Constructive characteristics of materials were observed by HE, collagen stain and scanning electron microscope. All materials were implanted subcutaneously in rabbits and extirpated at 4, 6, 8, 10 weeks respectively to study their biocompatibility and biodegradibility. RESULTS Porous diameters of collagen membrane, PLGA and PHB were 107 μm, 179 μm and 13 μm respectively. All materials were well tolerated by the tissue and no abscess or tissue necrosis was observed. Mononuclear macrophage, fibroblast and fibrous proliferation were typical tissue response. Collagen membrane and PLGA were completely degraded at the end of 12 weeks. No degradation of PHB were observed at the end of 12 weeks. CONCLUSION Collagen membrane and PLGA meet the demands of scalffold of tissue engineered heart valve in its constructive characteristics, biocompatibility and biodegradation.

关 键 词：胶原 聚β羟基丁酯 聚乳酸聚乙醇酸 生物医学工程 心脏瓣膜 生物相容性 

分 类 号：R542.5[医药卫生—心血管疾病]