抗藻酸盐血清与加替沙星联合对铜绿假单胞菌生物被膜形态的影响  

Influence of anti-alginate serum combined with gatifloxacin on mucoid pseudomonas aeruginosa biofilm morphology

作　　者：王睿[1] 裴斐[1] 柴栋[1] 陈迁[1] 李新华[1] 

机构地区：[1]解放军总医院临床药理研究室,北京100853

出　　处：《中华医学杂志》2002年第18期1276-1278,共3页National Medical Journal of China

基　　金：国家自然科学基金资助项目 (3 0 0 70 72 0 )

摘　　要：目的　探讨抗藻酸盐血清对黏液型铜绿假单胞菌生物被膜中细菌形态上的影响。方法采用改良的组织培养平板法建立黏液型铜绿假单胞菌生物被膜模型 ,经不同稀释度的抗藻酸盐血清与不同浓度的加替沙星联合应用处理后 ,以银染法快速鉴定 ,扫描电镜 (SEM)确认细菌生物被膜形态 ,并采用计算机图像处理系统进行图像分析。结果　计算机图像分析系统显示 ,空白组生物被膜的平均光密度为 0 82± 0 0 6 ;加入抗藻酸盐血清后 ,抗藻酸盐血清组、抗藻酸盐血清与加替沙星联合用药组生物被膜的平均光密度值分别为 0 79± 0 0 6和 0 70± 0 0 4 ,加替沙星单独用药组生物被膜平均光密度值为 0 76± 0 0 7的 ;光镜和电镜图像可见黏液样物变稀疏 ,细菌数减少 ,形态不甚规则。结论抗藻酸盐血清能够影响黏液型铜绿假单胞菌生物被膜构建和形态 ,与加替沙星联用后可以使黏液型铜绿假单胞菌生物被膜结构发生不同程度破坏。Objective To investigate the influence of anti-alginate serum on morphology of biofilm (BF) of mucoid Pseudomonas aeruginosa. Methods The modified plate culture method was used to establish a biofilm model of mucoid Pseudomonas aeruginosa in vitro. The biofilm was treated by anti-alginate serum and/or different concentrations of gatifloxacin. The samples were then detected by scanning electron microscopy (SEM) and optical microscopy after being stained with AgNO 3. A group of samples treated by none agent was used as blank control. The optical density of bacterial biofilm was determined by using computer image analysis system. Results BF was achieved on the silicon slides in all groups 7 days after the incubation of Pseudomonas aeruginosa. The silicon slides were covered by dense and black stained material in the blank group; the black stained material as mildly less in the group treated with anti-alginate serum, and much less in the group treated with gatifloxacin, and the BF was thin and only slightly stained black in the group treated with anti-alginat combined with gatifloxacin. SEM showed that the mucoid materials among bacteria was reduced and the number of bacteria was decreased and the shape of bacteria became irregular in the groups treated by anti-alginate serun and/or gatiflaxacin, especially the group treated by anti-alginat combined with gatifloxacin. Computer image analysis system showed that the mean optical density was 0.82±0.06 in the blank control group, 0.79±0.06 in anti-alginate serum group, 0.76±0.07 in of gatifloxacin group, and 0.70±0.04 in anti-alginate serum combined with gatifloxacin group. Conclusion Anti-alginate serum affects the structure and morphology of the biofilm of mucoid Pseudomonas aeruginosa and destroys the biofilm structure when combined with gatifloxacin.

关 键 词：抗藻酸盐血清 加替沙星 生物膜 铜绿假单胞菌 

分 类 号：R965[医药卫生—药理学]