产超广谱β内酰胺酶产酸克雷伯菌的医院感染流行分析  

An outbreak of extended-spectrum beta-lactamase-producing Klebsiella oxytoca in a surgical ward

作　　者：崔岩[1] 沈定霞[1] 周贵民[1] 

机构地区：[1]解放军总医院微生物科,北京100853

出　　处：《中华检验医学杂志》2002年第5期277-280,共4页Chinese Journal of Laboratory Medicine

摘　　要：目的　研究同一时期、同一病房、不同病人临床分离的 8株多重耐药产酸克雷伯菌的同源特性。方法　对 8株产酸克雷伯菌进行药物敏感性试验、超广谱 β内酰胺酶 (ESBLs)表型测定、等电聚焦电泳、脉冲场凝胶电泳、聚合酶链反应 (PCR)及PCR产物测序等分子生物学分析。结果　 8株产酸克雷伯菌为产CTX M 3型ESBLs的亲缘关系密切的同源菌。结论　 8株产酸克雷伯菌在同一病房引起过小规模暴发流行 ,系同源菌。Objective To study 8 isolates of multiple antibiotic-resistant Klebsiella oxytoca in 8 different patients from one surgical ward. Methods Susceptibility test, extended-spectrum β-lactamases (ESBLs) screening methods and some molecular biological methods were performed. Results 8 isolates of Klebsiella oxytoca were verified as closely related homologous CTX-M-3-producing strains. Conclusions The little outbreak was caused by 8 closely related homologous strains in one ward.

关 键 词：医院感染 Β内酰胺酶类 克雷伯氏菌属 耐药性 分子生物学 同源性 

分 类 号：R181.3[医药卫生—流行病学]