谷胱甘肽抑制蒿甲醚的抗血吸虫作用(英文)  

Glutathione Inhibits the Antischistosomal Activity of Artemether

作　　者：翟自立[1] 焦佩英[1] 梅静艳[1] 肖树华[1] 

机构地区：[1]中国疾病预防控制中心寄生虫病预防控制所

出　　处：《中国寄生虫学与寄生虫病杂志》2002年第4期212-215,共4页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：青蒿素科技基金资助项目~~

摘　　要：目的　观察还原型谷胱甘肽 (GSH)对蒿甲醚伍用氯化血红素抗日本血吸虫作用的影响。　方法　将蒿甲醚、氯化血红素、GSH及谷胱甘肽耗竭剂 2 ,4 -二硝基氯苯 (CDNB)单一或伍用加入含 5周龄血吸虫的培养液内 ,温育2 4 h后 ,测定虫体的丙二醛含量 ,并观察培养至 96 h虫体存活情况。感染小鼠经蒿甲醚 30 0 mg/kg治疗后 6、12或 2 4h,测定虫体的 GSH水平。　结果　体外 ,蒿甲醚伍用氯化血红素作用血吸虫 2 4 h后 ,虫丙二醛含量明显升高。培养时间延长 ,虫陆续死亡。GSH对蒿甲醚伍用氯化血红素诱导血吸虫脂质过氧化及杀虫作用具有拮抗作用 ,CDNB则有增强作用。体内 ,蒿甲醚作用血吸虫 6～ 2 4 h,虫体内 GSH水平先降低再明显升高。　结论　 GSH可能在血吸虫防御蒿甲醚衍生的毒性过氧化物和自由基攻击中起重要作用。Objective To observe the effect of reduced glutathione (GSH) and glutathione depleting agent 1-chloro-2,4-dinitrobenzene (CDNB) on the susceptibility of adult Schistosoma japonicum to artemether (Art) in combination with hemin in vitro. . Methods. In vitro, malondialdehyde(MDA) levels were determined in five-week-old worms incubated without or with Art, hemin, GSH, and CDNB, either alone or in combination, for 24 h, and the remaining worms were continuously incubated up to 96 h for worm survival assessment. In vivo, GSH levels were determined in worms freshly recovered from mice 6, 12 and 24 h after treatment with Art 300 mg/kg. . Results . In vitro, GSH decreased the proportion of worms killed by Art plus hemin, but CDNB rendered the worms susceptible to the killing. The above-mentioned distinguishing features of GSH and CDNB were associated with their reverse effect on worm lipid peroxidation induced by Art-hemin system. In vivo, Art led to a slight decrease followed by a significant increase in the parasite GSH levels. . Conclusion . GSH might play an important role in the defense of the worms against Art-generated toxic peroxides and free radicals.

关 键 词：日本血吸虫 还原型谷胱甘肽 蒿甲醚 血红素 血吸虫病 

分 类 号：R532.21[医药卫生—内科学] R965[医药卫生—临床医学]