热休克蛋白70在正常和放射复合伤口愈合血管再生过程中表达及意义研究  

Studies on the changes and significance for heat shock protein 70 expression in angiogenesis in healing of single wounding and radiation-combined of wounding skin

作　　者：彭瑞云[1] 王德文[1] 高亚兵[1] 熊呈琦[1] 秦全红[1] 崔雪梅[1] 杨红[1] 谷庆阳[1] 夏国伟[1] 崔玉芳[1] 陈浩宇[1] 胡文华[1] 

机构地区：[1]北京放射医学研究所,100850

出　　处：《中华放射医学与防护杂志》2002年第4期272-274,共3页Chinese Journal of Radiological Medicine and Protection

基　　金：全军"九五"重点招标课题基金资助项目 (96Z0 0 8)

摘　　要：目的　探讨HSP70在正常和放射复合伤口愈合过程中表达变化规律及其意义。方法72只Wistar二级大鼠背部皮肤制作圆形伤口后以 2 5Gy6 0 Coγ射线局部照射 ,于伤后 2、5、10、15、2 1和 2 8d活杀取材 ,采用LSAB免疫组织化学技术研究HSP70基因表达及意义。结果　单纯创伤组于伤后 2d见新生血管内皮细胞浆内HSP70阳性 ;5d时其阳性的内皮细胞增多 ,强度增强 ;伤后 10d仍可见部分内皮细胞浆内阳性 ;伤后 15d后 ,HSP70逐渐减少。创伤 +照射组伤后 5d始见血管内皮细胞浆内弱阳性 ,10～ 15d时阳性 ,2 1d后阴性。结论　HSP70参与伤口愈合血管再生的过程 ,可能对血管再生起促进作用 ;辐射使血管内皮细胞表达HSP70减少可能是辐射延迟伤口愈合的原因之一。Objective\ To observe the expression of HSP70 in healing of single wounding and radiation\|combined wounding of skin and investigate its significance in capillary regeneration. Methods\ Altogether 72 Wistar rats were subjected to excision of a circular full\|thickness skin with the diameter of 1 5 cm on the dorsum.After wounded,these rats in radiation group were locally irradiated immediately with 25 Gy 60 Co γ\|rays.The skin specimens were obtained on days 2,5,10,15,21 and 28 after injury and the expression of HSP70 was investigated by means of immunohistochemistry. Results\ HSP70 expression was positive in the plasma of endothelial cells of newly\|formed capillaries on day 2, strong positive in lot of these cells on day 5, positive in part of endothelial cells on day 10,and decreased on day 15 after wounding in the single wounding group,whereas in the radiation combined wounding group HSP70 was slightly positive in the endothelial cells on day 5,positive on days 10\|15,and negative after day 21. Conclusion\ HSP70 plays an important role in the course of capillary regeneration.It could accelerate angiogenesis.HSP70 expression decreased by radiation might be one of reasons for delayed healing of radiation\|combined wound.

关 键 词：放射复合伤 伤口愈合 电离辐射 血管再生 热休克蛋白70 免疫组化 

分 类 号：R818[医药卫生—放射医学]