昆布多糖的提取分离及F_1的纯化与鉴定  

Studies on extraction and isolation of polysaccharides from laminaria and purification and identification of F_1

作　　者：杜斌[1] 韩华云[2] 张秋荣[1] 

机构地区：[1]郑州大学药学系,河南郑州450052 [2]郑州大学分析测试中心,河南郑州450052

出　　处：《中国药学杂志》2002年第9期657-659,共3页Chinese Pharmaceutical Journal

摘　　要：目的　对昆布多糖的提取分离及F1的纯化、鉴定与免疫活性研究。方法　用热水从海带中提取昆布多糖 ,对粗多糖分级 ,对其中级分F1进一步纯化 ,进行组成分析和免疫活性实验。结果　F1经SephadexG 2 0 0柱色谱及纸色谱检查为单一组分 ,测其相对分子质量约 1.8× 10 4。结论　F1的单糖组成为岩藻糖、半乳糖、木糖。巨噬细胞吞噬实验表明 。OBJECTIVE To study the extraction of polysaccharides from Laminaria, and the isolation,purification and identification of F 1 and its immunnoacitivity.METHODS Polysaccharides was extracted with boiling water,their impurities were graded with purification and component analysis.RESULTS F 1 was found to be amylopectin by filtration on sephadex G 200.The molecular weight( r) of F 1 was estimated to be 1.8×10 4.CONCLUSION A soluble polysaccharide(F 1) was composed of fucose,gal and xyloes.Peritoneal macrophages could be markedly activated by F 1.

关 键 词：F1 昆布多糖 鉴定 免疫活性 海带 有效成分提取 有效成分分离 

分 类 号：R284.2[医药卫生—中药学]