bFGF促进内皮细胞在聚氨酯材料上黏附的机理探讨  

A Study on the Mechanism of bFGF Promoting Endothelial Cells to Adhere to Polyurethane Material

作　　者：周序珑 邓漪平[2] 谢隆初[3] 

机构地区：[1]暨南大学医学院病理教研室,广州510632 [2]中山医科大学组胚教研室,广州510089 [3]暨南大学水生生物研究所,广州510632

出　　处：《生物医学工程学杂志》2002年第3期386-388,共3页Journal of Biomedical Engineering

摘　　要：b FGF能够促进内皮细胞在人工聚氨酯材料上的黏附 ,但其分子机制尚不清楚。本文采用 Western Blot结合图像处理分析方法 ,观察了 b FGF对血管内皮细胞 β1整合素表达的影响 ,结果显示 b FGF处理组和对照组平均灰度值分别为 16 6 .11± 9.86和 175 .32± 5 .12 ,两组免疫酶显色有明显差异 ,表明 b FGF能够诱导内皮细胞膜表面 β1整合素合成增加。我们推测 b FGF通过上调内皮细胞 β1整合素表达 ,增强了内皮细胞在聚氨酯材料上的黏附。本文提示 b FGF是实现人工心。bFGF can promote endothelial cell to adhere to artificial polyurethane material. However the mechanism is still unclear. Here the effect of bFGF on endothelial cell expression of integrin β 1 subunits was determined with Western Blot and image analysis method. The result reveal that the mean gray value of immunostain is 166.1±9.86 in the experimental group and 175.32±5.12 in the control group , suggest that bFGF may upregulate the endothelial cell expression of integrin β 1 subunits. It is concluded that the bFGF may induce the overexpression of endothelial cell integrin β 1 subunits to increase the cell adhesion onto polyurethane material. It is suggested that bFGF can be taken as an important assistant factor in accomplishment of endothelialization on artificical heart and blood vessel.

关 键 词：碱性成纤维细胞生长因子 聚氨酯 整合素 内皮细胞化 细胞粘附 人工心 人工血管 

分 类 号：R318.08[医药卫生—生物医学工程]