体外用条件培养液诱导脐带血间充质干细胞分化成心肌细胞的实验研究  

The Differentiation of Mesenchymal Stem Cells in Cord Blood into Cardiomyocytes in the Conditioned Medium in Vitro

作　　者：何培根[1] 余正堂[1] 

机构地区：[1]郧阳医学院附属东风医院心内科,湖北十堰442008

出　　处：《郧阳医学院学报》2002年第4期196-198,共3页Journal of Yunyang Medical College

摘　　要：目的 :探讨体外用条件培养液诱导脐带血间充质干细胞分化成心肌细胞的可行性。方法 :用水囊法引产的胎儿心肌制备条件培养液 ,以Ficoll作为离心介质分离脐带血中单个核细胞 (MNC) ,用贴壁法分离出间充质干细胞 (MSCs) ,移入条件培养液中培养 ,已培养的细胞在第 2 0天用心肌特有的抗肌球蛋白重链 (MHC)抗体作免疫组化。结果 :经条件培养液培养的细胞约 2 5 %免疫组化呈阳性 ,对照组均为阴性。结论Objective To investigate the feasibility of forming cardiomyocytes by inducing mesenchymal stem cells(MSCs) of cord blood in the conditioned medium in vitro.Methods Mononuclear cells (MNC) were taken from cord blood of the placenta by Ficoll. MSCs were obtained by adhering wall. Myocardium was cultered from fetus induced labor by water filled balloon method to provide conditioned medium , in which MSCs of cord blood were incubated. On the 20th day, the cultured cells were immunohistochemically stained for myosin heavy chain (MHC) .Results 25% of cultured cells in the conditioned medium formed myotubes which were stained positively for myosin heavy chain(MHC) , the control was 0%. Conclusion MSCs of cord blood can be induced and differentiated into cardiomyocytes in the conditioned medium in vitro.

关 键 词：条件培养液 脐带血 间充质干细胞 肌球蛋白重链 心肌细胞 实验研究 

分 类 号：R541.6[医药卫生—心血管疾病]