何氏凤仙(Impatiens holstii)在试管内诱导开花时的蛋白变化  

The Change of Proteins in Introducing Flowering of Impatiens holstii in vitro

作　　者：余沛涛[1] 周伟明[1] 

机构地区：[1]上海师范大学生命与环境科学学院,上海200234

出　　处：《上海师范大学学报（自然科学版）》2002年第3期57-60,共4页Journal of Shanghai Normal University(Natural Sciences)

基　　金：上海市教委资助项目 (项目编号 0 1D0 7)

摘　　要：在诱导何氏凤仙 (Impatiensholstii)试管内开花时 ,培养基中N的含量对开花有一定的影响 ,在培养基中的绝对N含量在 3 1 3 .65～ 62 7.3 (mg/L) ,或相对N含量为 61 .8‰ 97.9‰时 ,有利于开花。与MS相比 ,在开花率相同的培养基中 ,N的绝对含量相差很大 ,而相对含量却在同一水平 ,而且 ,相对N含量的变化 ,与开花呈相关性变化。在诱导开花和不诱导开花的植株中 ,2 0kD到 40kD之间的蛋白质有明显差异 ,这显示有新物质产生 ,也显示此诱导的开花过程是从代谢的上游开始的。The Impatiens holstii in vitro was favourable for blooming when cultured in mediums which N content was 313.65~627.3(mg/L) or 61.8‰～97.9‰. Comparing with that cultivated in medium of MS, when bloom level was the same, the net content of N was different very much, while the relative content of N was in the same level. Cultured in mediums with different contents of N, 25‰ and 75.8‰ respectively, after 4 weeks, with blooming or no blooming, there was notable difference protein in 20000D and 40000D in Impatiens holstii in vitro.

关 键 词：何氏凤仙 试管内开花 N含量 蛋白质 培养基 开花率 成花机理 

分 类 号：S681.1[农业科学—观赏园艺] Q945.64[农业科学—园艺学]