抗CD4、CD8单抗诱导异基因骨髓移植嵌合供体小鼠免疫耐受  

Induction of chimerism donor mouse immune tolerance to allogeneic bone marrow transplantation by anti-CD4 and CD8 monoclonal antibody

作　　者：朱彦[1] 罗鸣[1] 巴荣[1] 费小明[1] 金蓉珍[1] 

机构地区：[1]镇江医学院附属医院血液科,212001

出　　处：《江苏医药》2002年第10期743-744,共2页Jiangsu Medical Journal

摘　　要：目的　探讨诱导骨髓移植物的免疫耐受。方法　通过尾静脉给ICR系小鼠注入抗CD4、CD8单抗和同种异基因昆明系小鼠的骨髓 ,使之成为嵌合体小鼠。最后将该嵌合体小鼠的骨髓移植到宿主体内。结果　 ,实验组和对照组的植活皮片中位累计生存率分别为 10 0 %和 33 33% ,经Wilcoxon检验P <0 0 5。结论　抗CD4、CD8单抗与异基因骨髓细胞的联合应用可诱导供体产生特异性免疫耐受 。Objective To induce bone marrow grafting tolerance.Methods Anti CD4 and anti CD8 McAb were injected intravenously to ICR mice chimed by the bone marrow of Kunming mice.The bone marrow of the chimerism mice was grafted into the host mice.Results Comparison of survival experience using the Wilcoxon(Gehan) statistic survival variable day:control group is 33 33%;Experimental group is 100%.Conclusion The difference of the survival experience between control group and experimental group is significant( P< 0 05).The result shows the anti CD4,anti CD8 McAb and allogeneic bone marrow can induce donor specific immune tolerance.After the bone marrow of the chimerism donor was grafted,survival of receptor can be prolonged.

关 键 词：单抗 异基因骨髓移植 免疫耐受 动物实验 移植物抗宿主病 GVHD 

分 类 号：R392.4[医药卫生—免疫学] R457.7[医药卫生—基础医学]