低浓度铅暴露对鲫鱼肝脏抗氧化系统的影响  

EFFECT OF TRACE LEAD ON THE ANTIOXIDANT SYSTEM OF FISH LIVER

作　　者：陈亮[1] 郭红岩[1] 沈红[1] 王晓蓉[1] 

机构地区：[1]污染控制与资源化国家重点实验室,南京大学环境学院,南京210093

出　　处：《环境化学》2002年第5期485-489,共5页Environmental Chemistry

基　　金：教育部博士点基金资助项目 (编号 2 0 0 0 0 2 82 4 )

摘　　要：研究了鲫鱼幼体 (Carassiusauratus)在低浓度铅的长期 ( 4 0d)暴露实验中 ,鲫鱼抗氧化系统所产生的生理生化反应 .结果表明 ,在本实验的剂量范围内 ,铅对鲫鱼肝脏内过氧化氢酶 (CAT)、超氧化物歧化酶 (SOD)的活性 (在暴露浓度达到 0 2mg·l- 1时 )表现为诱导作用 ;而铅对谷胱甘肽硫转移酶 (GST)、谷胱甘肽过氧化物酶 (GSH Px)的活性在低浓度时 ( 0 0 1mg·l- 1)被抑制 ,其中暴露浓度在 0 2mg·l- 1时GSH Px被进一步抑制 .在实验范围内 ,GSH含量无明显变化 .另外 ,对于低浓度铅的长期暴露 ,鲫鱼肝脏中的GST和GSH Px较CAT和SOD敏感 ,可考虑作为水生生态系统中铅低浓度长期暴露的一种生物检测指标 .The aim of this work was to study the effects of trace lead under long term exposure on fish antioxidant system,such as the activities of SOD,CAT,GSH-Px,GST and the content of GSH.During 40 days exposure,we found that the activities of SOD and CAT increased significantly when the concentration of Pb 2+ reach 0.2 mg·l -1 ,whereas the activities of GST and GSH-Px decreased significantly at 0.01mg·l -1 Pb 2+ and GSH-Px activity decreased again at 0.2 mg·l -1 Pb 2+ ,no obvious change of GSH content in the fish liver was found.These results suggest that GST and GSH-Px are more sensitive than CAT and SOD to low dose lead exposure,and they could be served as bio-monitoring index of low concentration lead exposure in aquatic ecosystem.

关 键 词：低浓度 肝脏 铅 鲫鱼 抗氧化系统 生态毒理 生态监测 

分 类 号：X503[环境科学与工程—环境工程]