运动病大鼠脑细胞三种酶活性变化的图像定量分析  

Imagine quantitative analysis on activity of three kinds of enzyme in rat′s brain cells with motion sickness

作　　者：王琰[1,2] 徐根兴[1,2] 袁驾南 董文度[1,2] 金淑仪 庄坚[1,2] 姜正林[1,2] 李建成 

机构地区：[1]海军医学高等专科学校细胞生物学研究室 [2]南通医学院航海医学研究所

出　　处：《中国体视学与图像分析》1998年第2期65-67,共3页Chinese Journal of Stereology and Image Analysis

摘　　要：采用正负交变加速度旋转刺激制备大鼠运动病模型。对运动病大鼠脑细胞Ｎａ＋Ｋ＋ＡＴＰａｓｅ、碱性磷酸酶和细胞色素氧化酶活性变化进行图像定量分析。结果表明，运动病大鼠大脑皮质、小脑皮质和脑干前庭区脑细胞Ｎａ＋Ｋ＋ＡＴＰａｓｃ和细胞色素氧化酶活性明显降低，而碱性磷酸酶活性明显升高。提示运动病的发生与中枢神经细胞有氧代谢、细胞膜通透性和钠。Sixteen rats that were subjected to rotational stimulation engaged in pica were used as the experimental models. The activity variations of Na + K + ATPase and cytochrome oxidase were meassured by means of image quantitative analysis in brain cells of rats with motion sickness. It was showed that the activity of Na + K + ATPase and cytochrome oxidase decreased markedly in cerebral cortex, cerebellum cortex and vestibular area of brainstem, while alkaline phosphatase activity increased sharply. It is suggested that the development of motion sickness may be related to oxygenic metabolism, membrane permeability and Na + K + exchange.

关 键 词：运动病 ATP酶 碱性磷酸酶 细胞色素氧化酶 

分 类 号：R874[医药卫生—运动医学]