柯里拉京抗肿瘤作用的研究  

Experiment studies on the pharmacodynamics experiment by corilagin

作　　者：刘朝阳[1] 王德昌[1] 陈玉武[2] 任丽娟[2] 李克明[2] 张伟[1] 

机构地区：[1]中国医学科学院肿瘤研究所,北京100021 [2]北京中日友好医院临床医学研究所

出　　处：《肿瘤防治研究》2002年第5期356-358,共3页Cancer Research on Prevention and Treatment

基　　金：国家新药研究基金资助;国家重点科技项目(96 90 1 0 5 178)

摘　　要：目的　观察柯里拉京 (Corilagin)体内、外抗肿瘤活性及其量效关系。 方法　体外药效试验用MTT法 ,体内用小鼠移植性肿瘤及裸鼠移植人肿瘤细胞株观察柯里拉京抑瘤活性及与其他化疗药的协同作用。结果　柯里拉京体外对人卵巢癌细胞 (A2 780 )、鼻咽癌细胞 (KB)、骨肉瘤细胞 (OS 732 )和人结肠癌细胞 (HCT 8)IC50 值分别为 0 .2 5 μg/ml、1.2 μg/ml、7.4 2 μg/ml及 9.0 8μg/ml。体内对小鼠移植性肿瘤及裸鼠移植人肿瘤细胞株也显示出较显著抑制作用。结论　柯里拉京体外对A2 780、KB、OS 732和HCT 8人结肠癌细胞有明显的细胞毒活性 ,体内对小鼠肝癌H2 2 ,裸鼠移植人OS 732、HCT 8也显示出较好的抑制作用。Objective To study the antitumor activity of Corilagin both in vitro and in vivo,and further evaluate its dose\|effect relationship.Methods In vitro and in vivo study on the pharmacological activity of Corilagin were performed by using MTT assay and mice transplant tumor model combined chemical agent respectively.Results IC 50 numerical value of A2780 cells,KB cells,OS\|732 cells,and HCT\|8 cells were 0.25 μg/ml, 1.2 μg/ml, 7.42 μg/ml, 9.08 μg/ml in vitro, respectively, in addition corilagin demonstrated antitumor activity in vivo.Conclusion Corilagin had not only obvious inhibitory tumor activity on human tumor cell strains such as A2780 cells, KB cells OS\|732 cells, HCT\|8 cells in vitro, but also demonstrated antitumor efficacy on mice transplant tumors such as H 22 ,OS\|732, and HCT\|8 strain in vivo.

关 键 词：柯里拉京 抗肿瘤作用 密柑草 体内抗瘤活性 量效关系 体外抗瘤活性 

分 类 号：R285.5[医药卫生—中药学]